Endogenous 5-HT outflow from chicken aorta by 5-HT uptake inhibitors and amphetamine derivatives
| Autor: | Dugar Delgermurun, Ken-ichi Otsuguro, Soichiro Yamaguchi, Shigeo Ito, Toshio Ohta |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Serotonin chicken Endogeny Aorta Thoracic Biology In Vitro Techniques Methamphetamine 03 medical and health sciences amphetamine derivative 0302 clinical medicine thoracic aorta medicine.artery Internal medicine Fluoxetine 5-HT outflow medicine Extracellular Thoracic aorta Animals P-Chloroamphetamine Serotonin Uptake Inhibitors p-Chloroamphetamine 5-HT receptor Pharmacology General Veterinary Full Paper 030104 developmental biology Endocrinology Nicotinic agonist Fluvoxamine Clomipramine Chickens 030217 neurology & neurosurgery Selective Serotonin Reuptake Inhibitors uptake inhibitor |
| Zdroj: | The Journal of Veterinary Medical Science |
| ISSN: | 1347-7439 |
| Popis: | Chemoreceptor cells aggregating in clusters in the chicken thoracic aorta contain 5-hydroxytryptamine (5-HT) and have voltage-dependent ion channels and nicotinic acetylcholine receptors, which are characteristics typically associated with neurons. The aim of the present study was to investigate the effects of 5-HT uptake inhibitors, fluvoxamine, fluoxetine and clomipramine (CLM), and amphetamine derivatives, p-chloroamphetamine (PCA) and methamphetamine (MET), on endogenous 5-HT outflow from the isolated chick thoracic aorta in vitro. 5-HT was measured by using a HPLC system with electrochemical detection. The amphetamine derivatives and 5-HT uptake inhibitors caused concentration-dependent increases in endogenous 5-HT outflow. PCA was about ten times more effective in eliciting 5-HT outflow than MET. The 5-HT uptake inhibitors examined had similar potency for 5-HT outflow. PCA and CLM increased 5-HT outflow in a temperature-dependent manner. The outflow of 5-HT induced by PCA or 5-HT uptake inhibitors was independent of extracellular Ca(2+) concentration. The 5-HT outflow induced by CLM, but not that by PCA, was dependent on the extracellular NaCl concentration. These results suggest that the 5-HT uptake system of 5-HT-containing chemoreceptor cells in the chicken thoracic aorta has characteristics similar to those of 5-HT-containing neurons in the mammalian central nervous system (CNS). |
| Databáze: | OpenAIRE |
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