Brachybacterium endophyticum sp. nov., a novel endophytic actinobacterium isolated from bark of Scutellaria baicalensis Georgi
| Autor: | Fei-Na Li, Yu-Xin Bao, Li Tuo, Hui-Chang Shi, Hong-Ying Li, Cheng-Hang Sun, Xiao-Rui Yan |
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| Rok vydání: | 2018 |
| Předmět: |
DNA
Bacterial 0106 biological sciences 0301 basic medicine China Peptidoglycan Diaminopimelic Acid 010603 evolutionary biology 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound Cell Wall Phylogenetics RNA Ribosomal 16S Actinomycetales Phospholipids Phylogeny Ecology Evolution Behavior and Systematics Base Composition biology Strain (chemistry) Fatty Acids Nucleic Acid Hybridization Vitamin K 2 Sequence Analysis DNA General Medicine 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques 030104 developmental biology chemistry visual_art Plant Bark visual_art.visual_art_medium Scutellaria baicalensis Bark Diaminopimelic acid |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 68:3563-3568 |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.003032 |
| Popis: | A Gram-positive, aerobic, coccus-shaped, non-spore-forming actinobacterium, designated strain M1HQ-2T, was isolated from a surface-sterilized bark of Scutellaria baicalensis Georgi collected from Guizhou, China and tested using a polyphasic approach to determine its taxonomic position. Strain M1HQ-2T grew at 4–37 °C (optimum, 30 °C), pH 5.0–11.0 (pH 8.0) and in the presence of 0–15 % (w/v) NaCl (1–3 %). Substrate mycelia and aerial mycelia were not formed, and diffusible pigments were not observed on any media tested. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain M1HQ-2T belonged to the genus Brachybacterium and had the highest 16S rRNA gene sequence similarity of 97.6 % to Brachybacteriumsquillarum M-6-3T. Strain M1HQ-2T contained MK-7 as the dominant menaquinone. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The polar lipids profile of strain M1HQ-2T contained diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified lipid. The predominant fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0. The DNA G+C content of strain M1HQ-2T was 71.0 mol%. The average nucleotide identity value between strain M1HQ-2T and type strain of Brachybacterium sacelli was 76.7 %. The estimated DNA–DNA hybridization value between strain M1HQ-2T and type strain of B. sacelli was 20.6 %. On the basis of phylogenetic analysis, chemotaxonomic characteristics and phenotypic data, strain M1HQ-2T represents a novel species of the genus Brachybacterium , for which the name Brachybacterium endophyticum sp. nov. is proposed. The type strain is M1HQ-2T (=KCTC 49087T=CGMCC 1.16391T). |
| Databáze: | OpenAIRE |
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