Ouabain promotes immune responses in WEHI-3 cells to generate leukemia mice through enhancing phagocytosis and natural killer cell activities in vivo
| Autor: | Hsiao Min Chou, Ming Zhe Lee, Mei Hui Lee, Ying Ying Chuang, Yung Luen Shih, Kuo Wei Chen, Hsin Tu Hou, Shu Ching Hsueh, Hung Sheng Shang, Jing Gung Chung, Yung Liang Chen, Hsu Feng Lu |
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| Rok vydání: | 2018 |
| Předmět: |
Cytotoxicity
Immunologic Health Toxicology and Mutagenesis Phagocytosis 010501 environmental sciences Management Monitoring Policy and Law Pharmacology Toxicology Lymphocyte Activation 01 natural sciences Peripheral blood mononuclear cell CD19 Ouabain Natural killer cell 03 medical and health sciences Mice 0302 clinical medicine Immune system Cell Line Tumor medicine Macrophage Animals 0105 earth and related environmental sciences Mice Inbred BALB C Leukemia Experimental biology Chemistry General Medicine medicine.disease Antineoplastic Agents Phytogenic Killer Cells Natural Leukemia medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Neoplasm Transplantation medicine.drug |
| Zdroj: | Environmental toxicology. 34(5) |
| ISSN: | 1522-7278 |
| Popis: | Ouabain, a cardiotonic steroid, was used for the treatment of heart failure and atrial fibrillation and induces cancer cell apoptosis in many human cancer cells including human leukemia cells. However, there are no reports to show the effects on immune responses in a leukemia mouse model. In this study, WEHI-3 cell generated leukemia mice were developed and treated by oral ouabain at 0, 0.75, 1.5, and 3 mg/kg for 15 days. Results indicated that ouabain did not affect body appearance, but decreased liver and spleen weights, B- and T-cell proliferation at all three doses treatment and increased CD19 cells at 3.0 mg/kg treatment, decreased CD3, CD11b, and Mac-3 cells levels compared with positive control. Furthermore, ouabain increased the macrophage phagocytosis from peripheral blood mononuclear cell and peritoneal cavity at all three doses treatment and increased NK cell activities. Ouabain restored GOT, GPT and LDH levels in WEHI-3 leukemia mice in vivo. |
| Databáze: | OpenAIRE |
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