An ultrasound assisted extraction–solid‐phase extraction–ultra‐performance liquid chromatography combined strategy for atropine determination in Atropa belladonna leaves
| Autor: | Marí Castro Santos, Natally S.B. Toson, Thamires A. da Silva, Luiz C. Klein-Junior, Amélia T. Henriques, Mariana Koetz |
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| Rok vydání: | 2020 |
| Předmět: |
Atropine
Clinical Biochemistry Muscarinic Antagonists 030226 pharmacology & pharmacy 01 natural sciences Biochemistry High-performance liquid chromatography Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Atropa belladonna Drug Discovery Solid phase extraction Response surface methodology Molecular Biology Chromatography High Pressure Liquid Pharmacology Chromatography Plant Extracts Chemistry Alkaloid Solid Phase Extraction 010401 analytical chemistry Extraction (chemistry) Fractional factorial design General Medicine 0104 chemical sciences Plant Leaves Solvents Titration |
| Zdroj: | Biomedical Chromatography. 35 |
| ISSN: | 1099-0801 0269-3879 |
| Popis: | Atropine is an antimuscarinic alkaloid identified in Atropa belladonna. In pharmacopeias, percolation is standardized as an extraction method for A. belladonna leaves, along with liquid-liquid extraction as a cleanup procedure and titration as an analytical method for assaying the atropine in the leaves. In this study, a faster, solvent-saving, and more reliable method for quality control of A. belladonna samples was developed. Ultrasound-assisted extraction was proposed and optimized by fractional factorial design followed by Box-Behnken design. For modeling atropine content, the following optimal conditions were established: particle size, 180 μm; percentage methanol in water, 50%; volume of solvent, 15 ml; time of extraction, 60 min; and number of extractions, two. This led to a significant improvement in atropine extraction (P |
| Databáze: | OpenAIRE |
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