Activity- and reactivity-based proteomics: Recent technological advances and applications in drug discovery
| Autor: | Henry J Benns, Edward W. Tate, Ceire Joanne Wincott, Matthew A. Child |
|---|---|
| Přispěvatelé: | Biotechnology and Biological Sciences Research Council |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
0301 basic medicine Activity-based probes Druggability Computational biology Biology 0601 Biochemistry and Cell Biology 010402 general chemistry 01 natural sciences Biochemistry Analytical Chemistry 03 medical and health sciences Drug Discovery Humans Molecular Targeted Therapy Reactivity-based protein profiling 0304 Medicinal and Biomolecular Chemistry Drug discovery Organic Chemistry Chemical proteomics Activity-based proteomics 0104 chemical sciences Target identification and validation Protein profiling 030104 developmental biology Proteome Activity-based protein profiling |
| Zdroj: | Current Opinion in Chemical Biology. 60:20-29 |
| ISSN: | 1367-5931 |
| Popis: | Activity-based protein profiling (ABPP) is recognized as a powerful and versatile chemoproteomic technology in drug discovery. Central to ABPP is the use of activity-based probes to report the activity of specific enzymes or reactivity of amino acid types in complex biological systems. Over the last two decades, ABPP has facilitated the identification of new drug targets and discovery of lead compounds in human and infectious disease. Furthermore, as part of a sustained global effort to illuminate the druggable proteome, the repertoire of target classes addressable with activity-based probes has vastly expanded in recent years. Here, we provide an overview of ABPP and summarise the major technological advances with an emphasis on probe development. |
| Databáze: | OpenAIRE |
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