Cytokinin vectors mediate marker-free and backbone-free plant transformation
| Autor: | Marina Kalyaeva, Artesia Stivison, Craig Richael, Robert Chretien, Hua Yan, J. Troy Weeks, Caius M. Rommens, Sathya Adimulam |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Base Sequence
biology Agrobacterium Genetic Vectors fungi food and beverages Genetically modified crops Plants Genetically Modified biology.organism_classification Cell biology Genetically modified organism Tissue culture Transformation (genetics) Transformation Genetic Botany Genetics Animal Science and Zoology Vector (molecular biology) Agronomy and Crop Science Gene Solanaceae Selectable marker DNA Primers Rhizobium Biotechnology |
| Zdroj: | Transgenic Research. 17:905-917 |
| ISSN: | 1573-9368 0962-8819 |
| Popis: | Conventional Agrobacterium-mediated transformation methods rely on complex and genotype-specific tissue culture media for selection, proliferation, and regeneration of genetically modified cells. Resulting transgenic plants may not only contain selectable marker genes but also carry fragments of the vector backbone. Here, we describe a new method for the production of transgenic plants that lack such foreign DNA. This method employs vectors containing the bacterial isopentenyltransferase (ipt) gene as backbone integration marker. Agrobacterium strains carrying the resulting ipt gene-containing "cytokinin" vectors were used to infect explants of various Solanaceous plant species as well as canola (Brassica napus). Upon transfer to hormone-free media, 1.8% to 9.9% of the infected explants produced shoots that contained a marker-free T-DNA while lacking the backbone integration marker. These frequencies often equal or exceed those for backbone-free conventional transformation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink