Potentiation of antibiotic activity of aminoglycosides by natural products from Cordia verbenacea DC
| Autor: | Cassio R. Medeiros, Celestina E.S. Souza, José Galberto Martins da Costa, Maria Karollyna do Nascimento Silva, Erivania F. Alves, Vanessa de Carvalho Nilo Bitu, Aline Augusti Boligon, Margareth Linde Athayde, Fernando Gomes Figueredo, Henrique Douglas Melo Coutinho, Francisco A. V. Santos, Edinardo F.F. Matias, Victoria Regina de Alencar Carvalho |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Staphylococcus aureus medicine.drug_class Phytochemicals 030106 microbiology Antibiotics Ethyl acetate Microbial Sensitivity Tests medicine.disease_cause Microbiology Toxicology 03 medical and health sciences Minimum inhibitory concentration chemistry.chemical_compound Escherichia coli medicine Medicinal plants Chromatography High Pressure Liquid Biological Products Cordia Traditional medicine Chemistry Pseudomonas aeruginosa Drug Synergism Anti-Bacterial Agents Plant Leaves Aminoglycosides 030104 developmental biology Infectious Diseases Phytochemical Antibacterial activity |
| Zdroj: | Microbial Pathogenesis. 95:111-116 |
| ISSN: | 0882-4010 |
| Popis: | Medicinal plants are often the only therapeutic resource for many communities and ethnic groups. Cordia verbenacea DC., "Erva-baleeira," is one of the species of plants currently used to produce a phytotherapeutic product extracted from its leaves. The present study aimed to establish its chemical profile, antibacterial activity and resistance-modulating potential. The C. verbenacea extracts were prepared from fresh leaves using solvents as methanol and hexane. Ethyl Acetate was used for the preparation of the fraction. Phytochemical screening was carried out using HPLC-DAD for determination and quantification of the secondary metabolites present in the fractions. Antibacterial and resistance-modulation assays were performed to determine minimum inhibitory concentration (MIC) using a microdilution assay. The data were subjected to statistical analysis with two-way ANOVA and Bonferroni posttests. Results of phytochemical prospecting and HPLC analysis of the fractions were in agreement with the literature. The natural products presented moderate antibacterial activity when considering the clinical relevance of a MIC of 256 μg/mL against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa, and 512 μg/mL against P. aeruginosa. However, when the fractions were combined with antibiotics we observed a synergic effect, as natural products enhanced the antibacterial effect of aminoglycosides, significantly decreasing the MIC of antibiotics at 12.5%-98.4%. We believe that the data obtained from phytochemical analysis and from antibacterial and resistance modulation assays of C. verbenacea extracts new can open perspectives in the search for new alternatives for the treatment of bacterial infections and stimulate the renewed use of antibiotics with reduced effectiveness due to resistance. |
| Databáze: | OpenAIRE |
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