Comparing the effects of mitochondrial targeted and localized antioxidants with cellular antioxidants in human skin cells exposed to UVA and hydrogen peroxide
| Autor: | Anne O. Oyewole, Mark A. Birch-Machin, Marie-Claire Wilmot, Mark Ian Fowler |
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| Rok vydání: | 2013 |
| Předmět: |
DNA damage
Ubiquinone Ultraviolet Rays Oxidative phosphorylation Mitochondrion medicine.disease_cause Biochemistry Antioxidants chemistry.chemical_compound Organophosphorus Compounds Polymer chemistry Genetics Medicine Humans Molecular Biology Skin chemistry.chemical_classification MitoQ Tiron Reactive oxygen species business.industry Hydrogen Peroxide Molecular biology Mitochondria Comet assay chemistry 1 2-Dihydroxybenzene-3 5-Disulfonic Acid Disodium Salt Comet Assay business Oxidative stress Biotechnology DNA Damage |
| Zdroj: | FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 28(1) |
| ISSN: | 1530-6860 |
| Popis: | Skin cancer and aging are linked to increased cellular reactive oxygen species (ROS), particularly following exposure to ultraviolet A (UVA) in sunlight. As mitochondria are the main source of cellular ROS, this study compared the protective effects of mitochondria-targeted and -localized antioxidants (MitoQ and tiron, respectively) with cellular antioxidants against oxidative stress-induced [UVA and hydrogen peroxide (H2O2)] mitochondrial DNA (mtDNA) damage in human dermal fibroblasts. With the use of a long quantitative PCR assay, tiron (EC50 10 mM) was found to confer complete (100%) protection (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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