Granulocyte–macrophage colony‐stimulating factor induces the differentiation of murine erythroleukaemia cells into dendritic cells
| Autor: | Joseph Wang, Xuetao Cao, Yan Wang, Mingjian Zhang, Yongwei Yu, Y Zhao, Wannian Zhang |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Male
medicine.medical_treatment Immunology Active immunotherapy Biology Lymphocyte Activation Cancer Vaccines Immunophenotyping Mice Immune system medicine Animals Immunology and Allergy Cell adhesion Cell Line Transformed Mice Inbred BALB C Granulocyte-Macrophage Colony-Stimulating Factor Cancer Cell Differentiation Dendritic Cells Immunotherapy Flow Cytometry medicine.disease Adoptive Transfer Recombinant Proteins Cell biology Mice Inbred C57BL Microscopy Electron CTL Granulocyte macrophage colony-stimulating factor Leukemia Erythroblastic Acute Lymphocyte Culture Test Mixed Biomarkers T-Lymphocytes Cytotoxic Research Article medicine.drug |
| Zdroj: | Immunology. 95:141-147 |
| ISSN: | 1365-2567 0019-2805 |
| Popis: | Dendritic cells (DC) are professional antigen-presenting cells (APC) within the immune system and antigen-pulsed DC can be used as an effective vaccine for active immunotherapy of cancer. Granulocyte-macrophage colony-stimulating factor (GM-CSF) plays an important role in the generation of DC. We previously showed that GM-CSF can induce murine erythroleukaemia cells (FBL-3) to differentiate into monocyte-like cells. To develop a new vaccinating method to stimulate the host immune response to leukaemia, we further investigate whether FBL-3 cells induced by GM-CSF can differentiate into DC in the present study. After being treated with GM-CSF, FBL-3 cells expressed high levels of 33D1 and NLDC-145, which are the specific markers of DC. The expression of MHC-II, B7-1, B7-2 and vascular cell adhesion molecule-1 (VCAM-1) was up-regulated markedly; the typical morphology of DC were also observed by electron microscopy. Functionally, the GM-CSF-induced FBL-3 cells could apparently stimulate the proliferation of naive allogeneic and autologous T lymphocytes and induce the generation of specific CTL more efficiently than the wild-type FBL-3 cells. Mice immunized with GM-CSF-induced FBL-3 cells could resist the subsequent challenge with the wild-type FBL-3 cells. Collectively, these data indicate that GM-CSF differentiates murine erythroleukaemia cells into DC phenotypically, morphologically and functionally. FBL-3-derived DC can be used as a new type of vaccine. Our results may have important implications for the immunotherapy of leukaemia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|