Ceramic composite with gentamicin decreases persistent infection and increases bone formation in a rat model of debrided osteomyelitis
| Autor: | Marjolein C. H. van der Meulen, Giorgio Perino, Mathias P.G. Bostrom, Xu Yang, Robert Chojnowski, Aleksey Dvorzhinskiy |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
medicine.drug_class medicine.medical_treatment Rat model Antibiotics Original Full-Length Article 03 medical and health sciences 0302 clinical medicine Medicine Orthopedics and Sports Medicine Bone formation Orthopedic surgery 030203 arthritis & rheumatology Bone growth 030222 orthopedics Debridement business.industry Osteomyelitis Histology medicine.disease Surgery Infectious Diseases Gentamicin business RD701-811 medicine.drug |
| Zdroj: | Journal of Bone and Joint Infection Journal of Bone and Joint Infection, Vol 6, Pp 283-293 (2021) |
| ISSN: | 2206-3552 |
| Popis: | Introduction: Current methods of managing osteomyelitic voids after debridement are inadequate and result in significant morbidity to patients. Synthetic ceramic void fillers are appropriate for non-infected bone defects but serve as a nidus of re-infection in osteomyelitis after debridement. CERAMENT G (CG) is an injectable ceramic bone void filler which contains gentamicin and is currently being evaluated for use in osteomyelitic environments after debridement due to its theoretical ability to serve as a scaffold for healing while eliminating residual bacteria after debridement through the elution of antibiotics. The goal of this study was to evaluate (1) the rate of persistent infection and (2) new bone growth of a debrided osteomyelitic defect in a rat model which has been treated with either gentamicin-impregnated ceramic cement (CERAMENT G) or the same void filler without antibiotics (CERAMENT, CBVF). Methods: Osteomyelitis was generated in the proximal tibia of Sprague Dawley rats, subsequently debrided, and the defect filled with either (1) CG (n=20), (2) CBVF (n=20), or (3) nothing (n=20). Each group was euthanized after 6 weeks. Infection was detected through bacterial culture and histology. Bone growth was quantified using microCT. Results: Infection was not detected in defects treated with CG as compared with 35 % of defects (7/20) treated with CBVF and 50 % (10/20) of empty defects (p=0.001). Bone volume in the defect of CG-treated rats was greater than the CBVF (0.21 vs. 0.17, p=0.021) and empty groups (0.21 vs. 0.11, p) at 6 weeks after implantation. Conclusions: Ceramic void filler with gentamicin (CERAMENT G) decreased the rate of persistent infection and increased new bone growth as compared to the same void filler without antibiotics (CERAMENT) and an empty defect in a rat model of debrided osteomyelitis. |
| Databáze: | OpenAIRE |
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