Development of a kit for the assay of haemagglutination inhibition antibodies to flaviviruses using formalinized goose erythrocytes
| Autor: | Kola M. Rao, Mitzi Nagarkatti, Prakash S. Nagarkatti |
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| Rok vydání: | 1980 |
| Předmět: |
Hemagglutination Inhibition Tests
Erythrocytes Hemagglutination Preservation Biological Biology Antibodies Viral Microbiology Blood serum Goose Formaldehyde biology.animal Geese medicine Animals Flavivirus Public Health Environmental and Occupational Health food and beverages General Medicine Haemolysis biology.organism_classification medicine.disease Virology Hemolysis Infectious Diseases biology.protein Parasitology Antibody |
| Zdroj: | Transactions of the Royal Society of Tropical Medicine and Hygiene. 74:22-25 |
| ISSN: | 0035-9203 |
| Popis: | Haemagglutination inhibition (HI) test using goose erythrocytes (GRBC) is the method of choice for screening antibodies to various arboviruses. This test makes use of fresh GRBC preserved in dextrose-gelatin-veronal (DGV). We have tried formalinizing GRBC and have found that such cells can retain the surface property of being agglutinated by arboviruses and, therefore, can be used in haemagglutination (HA) and HI tests. Unlike fresh cells preserved in DGV, formalinized cells can be preserved for a long time without haemolysis or loss of sensitivity. They can also be frozen or lyophilized. Making use of these properties, a kit has been developed for the assay of HI antibodies to some members of the flavivirus group. Use of this kit simplifies the HI test and enables it to be carried out routinely by any hospital or laboratory to screen arboviral infections. |
| Databáze: | OpenAIRE |
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