Structural chromosome aberrations cause swelling of the nucleus
| Autor: | Kenji Takeshita, Toshinari Maeda, Hiroaki I. Ogawa |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Social Psychology Aneugen Environmental Science (miscellaneous) Biology medicine.disease_cause Chromosomal aberration 03 medical and health sciences Clastogen 0302 clinical medicine Genetics medicine Fibroblast Carcinogen Nuclear swelling Research Non-mutagen Methane sulfonate Cell cycle Molecular biology 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Interphase Genotoxicity |
| Zdroj: | Genes and Environment |
| ISSN: | 1880-7062 |
| DOI: | 10.1186/s41021-016-0047-7 |
| Popis: | Background Carcinogens are known to cause swelling of the mammalian cell nucleus. However, the mechanism of the swelling and its toxicological significance have not been fully elucidated. Since nuclear swelling (NS hereafter) has been frequently observed in chromosomal aberration (CA hereafter) tests (in vitro), the relationship between NS and CAs was investigated in this study. Results In a short-term CA test using the fibroblast CHL cell line, the appearance of NS increased in a dose-dependent manner after exposure to six types of clastogens (mitomycin C, methyl methane sulfonate, 1-methyl-3-nitro-1-nitrosoguanidine, benzo[a]pyrene, cyclophosphamide monohydrate, and 9,10-dimethyl-2-benzanthracene), and a strong correlation was found between NS (%) and CAs (%) at each dosage. Therefore, we hypothesized that clastogens cause NS in cultured mammalian cells, since the mouse lymphoma L5178Y cell line is known to have a similar sensitivity to clastogens. Thus, we measured NS for 14 compounds (clastogens) that are known to induce structural CAs, 4 aneugens, and 12 non-mutagenes. Almost all clastogens caused NS of more than 5 %, which increased in a dose-dependent manner. Among the aneugens, colchicine, and diethylstilbestrol caused the same level of NS % as the clastogens, while carbendazim and trichlorfon caused a similar level of NS % as the clastogens only at higher levels of cytotoxicity. Almost all the non-mutagens caused less than 5 % NS. Conclusions These results strongly suggest that NS is mainly caused by structural aberrations in the nucleus during interphase of the cell cycle. |
| Databáze: | OpenAIRE |
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