Paraburkholderia nodosa is the main N2-fixing species trapped by promiscuous common bean (Phaseolus vulgaris L.) in the Brazilian 'Cerradão'
| Autor: | Renata Carolini Souza, Lionel Moulin, Rebeca Fuzinatto Dall'Agnol, Ieda de Carvalho Mendes, Fábio Bueno dos Reis Junior, Fábio Plotegher, Mariangela Hungria, Gilles Béna |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Bacterial food.ingredient 030106 microbiology Biology Applied Microbiology and Biotechnology Microbiology Rhizobia 03 medical and health sciences Soil food Phylogenetics Nitrogen Fixation RNA Ribosomal 16S Botany Symbiosis Phylogeny Soil Microbiology Phaseolus Ecology Phylogenetic tree food and beverages Betaproteobacteria Edaphic Sequence Analysis DNA Paraburkholderia biology.organism_classification 030104 developmental biology Nitrogen fixation Rhizobium Brazil Polymorphism Restriction Fragment Length |
| Zdroj: | FEMS microbiology ecology. 92(8) |
| ISSN: | 1574-6941 |
| Popis: | The bacterial genus Burkholderia comprises species occupying several habitats, including a group of symbionts of leguminous plants-also called beta-rhizobia-that has been recently ascribed to the new genus Paraburkholderia We used common bean (Phaseolus vulgaris L.) plants to trap rhizobia from an undisturbed soil of the Brazilian Cerrado under the vegetation type 'Cerradao'. Genetic characterization started with the analyses of 181 isolates by BOX-PCR, where the majority revealed unique profiles, indicating high inter- and intra-species diversity. Restriction fragment length polymorphism-PCR of the 16S rRNA of representative strains of the BOX-PCR groups indicated two main clusters, and gene-sequencing analysis identified the minority (27%) as Rhizobium and the majority (73%) as Paraburkholderia Phylogenetic analyses of the 16S rRNA and housekeeping (recA and gyrB) genes positioned all strains of the second cluster in the species P. nodosa, and the phylogeny of a symbiotic gene-nodC-was in agreement with the conserved genes. All isolates were stable vis-a-vis nodulating common bean, but, in general, with a low capacity for fixing N2, although some effective strains were identified. The predominance of P. nodosa might be associated with the edaphic properties of the Cerrado biome, and might represent an important role in terms of maintenance of the ecosystem, which is characterized by acid soils with high saturation of aluminum and low N2 content. |
| Databáze: | OpenAIRE |
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