The BOC ELISA, a ruminant-specific AMH immunoassay, improves the determination of plasma AMH concentration and its correlation with embryo production in cattle

Autor: Jean-Yves Picard, Nathalie di Clemente, Nassim Arouche, Danielle Monniaux, Bernard Vigier, Soazik P. Jamin, Richard L. Cate, Joëlle Taieb, Nathalie Josso
Přispěvatelé: Physiologie de l'Axe Gonadotrope (PAG U1133), Unité de Biologie Fonctionnelle et Adaptative (BFA (UMR_8251 / U1133)), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris Diderot - Paris 7 (UPD7), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7)
Rok vydání: 2015
Předmět:
Anti-Mullerian Hormone
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
endocrine system
medicine.medical_specialty
Coefficient of variation
müllerian-inhibiting substance
Embryonic Development
Enzyme-Linked Immunosorbent Assay
Sensitivity and Specificity
03 medical and health sciences
0302 clinical medicine
Food Animals
Ruminant
Internal medicine
anti-müllerian hormone
Follicular phase
medicine
Animals
immunoassay
Small Animals
Ovarian Function Tests
030304 developmental biology
Detection limit
0303 health sciences
030219 obstetrics & reproductive medicine
biology
medicine.diagnostic_test
Plasma samples
Equine
Chemistry
bovine
[SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology
Embryo
Anti-Müllerian hormone
biology.organism_classification
Endocrinology
Immunoassay
biology.protein
Cattle
Female
Animal Science and Zoology
Zdroj: Theriogenology
Theriogenology, Elsevier, 2015, 84 (8), pp.1397-1404. ⟨10.1016/j.theriogenology.2015.07.026⟩
Theriogenology, 2015, 84 (8), pp.1397-1404. ⟨10.1016/j.theriogenology.2015.07.026⟩
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2015.07.026
Popis: International audience; Plasma anti-Müllerian hormone (AMH) concentrations have been recently found to be predictive of the number of embryos recovered after FSH superovulatory treatment in the cow. However, the sensitivity of the Active Müllerian-inhibiting substance/AMH ELISA (ref. 10–14400; DSL-Beckman-Coulter) used to make these measurements in bovine plasma samples is low because it was developed to measure human AMH levels. To overcome this limitation, we developed an immunoassay specific for the bovine (B), ovine (O), and caprine (C) species, the bovine-ovine-caprine (BOC) ELISA. For this purpose, we produced recombinant bovine AMH for standardization, and we used monoclonal antibodies raised against bovine AMH, previously prepared by our laboratory. We evaluated the precision, accuracy, specificity, limit of detection, and functional sensitivity of the assay. The intra-assay coefficient of variation ranged between 3.4% and 11.3% for AMH concentrations between 23.68 and 1.74 ng/mL, and the interassay coefficient of variation ranged between 4.8% and 20.5% for concentrations between 25.53 and 1.42 ng/mL, respectively. The assay displayed a good linearity, had a detection limit of 0.4 ng/mL and a functional sensitivity of 1.4 ng/mL. It also cross-reacted with ovine and caprine AMHs. Both the mean and median AMH levels measured in 40 cow plasma samples using the BOC ELISA were approximately 44 fold higher than the mean and median AMH levels measured with the Active Müllerian-inhibiting substance/AMH ELISA. Moreover, a higher correlation was observed between the average number of embryos recovered from each cow after superovulatory treatment and AMH concentrations measured with the BOC ELISA. This BOC ELISA provides a very efficient tool for evaluating the ovarian follicular reserve of cows and predicting their embryo production capacity.
Databáze: OpenAIRE
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