Molecularly imprinted polymer based on MWCNT-QDs as fluorescent biomimetic sensor for specific recognition of target protein
| Autor: | Jing Quan, S. W. Annie Bligh, Ding Zhaoqiang, Li-Min Zhu, Lei Tao, Hua-Li Nie, Gong Xiao |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Materials science
Bioengineering Sensitivity and Specificity Biomaterials Hemoglobins chemistry.chemical_compound Biomimetic Materials Quantum Dots Animals Bovine serum albumin Detection limit Chromatography biology Nanotubes Carbon technology industry and agriculture Molecularly imprinted polymer Serum Albumin Bovine equipment and supplies Fluorescence Spectrometry Fluorescence chemistry Mechanics of Materials Quantum dot biology.protein Cattle Muramidase Target protein Lysozyme Luminescence |
| Zdroj: | Materials Science and Engineering: C. 48:469-479 |
| ISSN: | 0928-4931 |
| DOI: | 10.1016/j.msec.2014.12.032 |
| Popis: | A novel molecularly imprinted optosensing material based on multi-walled carbon nanotube-quantum dots (MWCNT-QDs) has been designed and synthesized for its high selectivity, sensitivity and specificity in the recognition of a target protein bovine serum albumin (BSA). Molecularly imprinted polymer coated MWCNT-QDs using BSA as the template (BMIP-coated MWCNT-QDs) exhibits a fast mass-transfer speed with a response time of 25 min. It is found that the BSA as a target protein can significantly quench the luminescence of BMIP-coated MWCNT-QDs in a concentration-dependent manner that is best described by a Stem-Volmer equation. The K-SV for BSA is much higher than bovine hemoglobin and lysozyme, implying a highly selective recognition of the BMIP-coated MWCNT-QDs to BSA. Under optimal conditions, the relative fluorescence intensity of BMIP-coated MWCNT-QDs decreases linearly with the increasing target protein BSA in the concentration range of 5.0 x 10(-7)-35.0 x 10(-7) M with a detection limit of 80 nM. |
| Databáze: | OpenAIRE |
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