Sensitivity of the Phadia EliA connective tissue disease screen for less common disease-specific autoantibodies
| Autor: | Jennifer C Parker, Christopher C. Bunn |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
biology
business.industry Autoantibody Enzyme-Linked Immunosorbent Assay General Medicine medicine.disease medicine.disease_cause Autoantigens Sensitivity and Specificity Connective tissue disease Autoimmune Diseases Pathology and Forensic Medicine Proliferating cell nuclear antigen Autoimmunity Antigen Immunology medicine biology.protein Humans CTD Antibody Connective Tissue Diseases business Polymerase Autoantibodies |
| Zdroj: | Journal of Clinical Pathology. 64:631-633 |
| ISSN: | 0021-9746 |
| DOI: | 10.1136/jcp.2010.084756 |
| Popis: | Aim To evaluate the sensitivity of a commercial autoantibody screening method (Phadia EliA connective tissue disease (CTD) screen) for the detection of less common antibody specificities associated with connective tissue disease. Methods 399 sera positive for anti-PM/Scl (n=102), anti-RNA polymerase III (n=199), anti-fibrillarin (n=50), anti-Mi-2 (n=12), anti-proliferating cell nuclear antigen (PCNA) (n=13) and anti-ribosomal P (n=23) were analysed using the solid phase assay. Each well was coated with the respective antigens for these autoantibodies and also with the antigens Ro, La, Jo-1, Scl-70, CENP-B, U1-RNP, Sm and native DNA. Results All the anti-ribosomal P, anti-PCNA and anti-Mi-2 sera and 94% of the anti-PM/Scl sera were positive. For anti-fibrillarin, 36 (68%) were positive and 12 (22%) were equivocal. For anti-RNA polymerase III, 131 (67%) were positive, 23 (11%) were equivocal and 45 (22%) were negative. Conclusions The sensitivity of the Phadia EliA CTD screen is currently insufficient for the assay to be used as a screening test for anti-fibrillarin and anti-RNA polymerase III, but appears to be satisfactory for the other autoantibodies tested. |
| Databáze: | OpenAIRE |
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