miR-200 Regulates Endometrial Development During Early Pregnancy
| Autor: | Carole R. Mendelson, Patricia T. Jimenez, W. Lee Kraus, R. Ann Word, Monica Mainigi |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Epithelial-Mesenchymal Transition Stromal cell Immunoblotting In Vitro Techniques Biology Andrology Endometrium Mice 03 medical and health sciences Endocrinology Pregnancy Internal medicine microRNA medicine Animals Humans Molecular Biology Cells Cultured Original Research Zinc finger Wound Healing Gene knockdown Reverse Transcriptase Polymerase Chain Reaction Decidualization Embryo General Medicine MicroRNAs 030104 developmental biology Female Stromal Cells Signal transduction Hormone |
| Zdroj: | Molecular Endocrinology. 30:977-987 |
| ISSN: | 1944-9917 0888-8809 |
| Popis: | For successful embryo implantation, endometrial stromal cells must undergo functional and morphological changes, referred to as decidualization. However, the molecular mechanisms that regulate implantation and decidualization are not well defined. Here we demonstrate that the estradiol- and progesterone-regulated microRNA (miR)-200 family was markedly down-regulated in mouse endometrial stromal cells prior to implantation, whereas zinc finger E-box binding homeobox-1 and -2 and other known and predicted targets were up-regulated. Conversely, miR-200 was up-regulated during in vitro decidualization of human endometrial stromal cells. Knockdown of miR-200 negatively affected decidualization and prevented the mesenchymal-epithelial transition-like changes that accompanied decidual differentiation. Notably, superovulation of mice and humans altered miR-200 expression. Our findings suggest that hormonal alterations that accompany superovulation may negatively impact endometrial development and decidualization by causing aberrant miR-200 expression. |
| Databáze: | OpenAIRE |
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