Addressable droplet microarrays for single cell protein analysis

Autor: David R. Klug, Ali Salehi-Reyhani, Oscar Ces, Keith R. Willison, Edward Burgin
Rok vydání: 2014
Předmět:
Zdroj: The Analyst. 139(21)
ISSN: 1364-5528
Popis: Addressable droplet microarrays are potentially attractive as a way to achieve miniaturised, reduced volume, high sensitivity analyses without the need to fabricate microfluidic devices or small volume chambers. We report a practical method for producing oil-encapsulated addressable droplet microarrays which can be used for such analyses. To demonstrate their utility, we undertake a series of single cell analyses, to determine the variation in copy number of p53 proteins in cells of a human cancer cell line.
Databáze: OpenAIRE