Apoptosis repressor with caspase recruitment domain (ARC) inhibits myogenic differentiation
| Autor: | Shirley C. Chen, Daryoush Ekhterae, David J. Granville, Jingchun Zhang, Honglin Luo, Arwen L. Hunter, Brian W. Wong, Xiaoning Si |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Caspase activation
Biophysics Gene Expression Muscle Proteins Repressor Apoptosis Muscle Development Transfection Biochemistry Cell Line Structural Biology Genetics medicine Animals Myocyte Myogenic differentiation Molecular Biology Myogenin Caspase Arc (protein) biology Caspase 3 Skeletal muscle Cell Differentiation Heart Cell Biology ARC Molecular biology Recombinant Proteins Rats Enzyme Activation medicine.anatomical_structure H9c2 cells Relative fluorescence units biology.protein Apoptosis Regulatory Proteins Myoblasts Cardiac |
| Zdroj: | FEBS Letters. 581:879-884 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/j.febslet.2007.01.050 |
| Popis: | Apoptosis repressor with caspase recruitment domain (ARC), an anti-apoptotic protein, is highly expressed in differentiated heart and skeletal muscle. Apoptosis and differentiation share numerous common pathways; therefore, we examined the impact of ARC on H9c2-myoblast differentiation. We demonstrate that ARC expression levels increase and stabilize upon differentiation. ARC-overexpression in pre-differentiated H9c2-cells suppresses differentiation; indicated by increased myotube formation, nuclear fusion and expression of the differentiation markers myogenin and troponin-T. ARC-overexpression inhibited myoblast differentiation associated caspase-3 activation, suggesting ARC inhibits myogenic differentiation through caspase inhibition. In summary, we show a novel role for ARC in the regulation of muscle differentiation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text