In vitro properties of small ruminant lentivirus genotype E
| Autor: | Maria Cristina Stella, M. Armentano, E. Bollo, Luigi Bertolotti, Ramsés Reina, Margherita Profiti, Sergio Rosati, Beatriz Amorena, M. Juganaru |
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| Rok vydání: | 2011 |
| Předmět: |
Genotype
viruses Protein subunit Population Virus Virology Animals Small ruminant Genotype E Viral pseudotype education Gene Cells Cultured Cell Proliferation Genetics education.field_of_study Goat Diseases Base Sequence Virulence biology Goats Macrophages Cell Cycle Lentivirus Synovial Membrane Entry assay Virus Internalization biology.organism_classification In vitro dUTPase Milk Choroid Plexus Small ruminant lentivirus vpr-like |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0042-6822 |
| Popis: | 8 p., 6 figures, 1 table and references Small ruminant lentivirus genotype E lacks the dUTPase subunit and vpr-like gene. Two strains (Roccaverano and Seui) with identical genetic organization have been described, with the env HV1–HV2 domains being the most divergent. Although dUTPase and vpr-like deletions have been involved in the RT fidelity in non dividing cells, both strains were able to replicate efficiently in blood derived macrophages (BDM), while virus production of E1 subtype was reduced or abrogated in replicating fibroblastic-like cells. The transcriptional activity of genotype E was similar in these two cellular populations. When viral pseudotypes were generated with the env of both viruses, Roccaverano pseudotype displayed a paranuclear localization on BDM, suggesting a different mechanism of entry. Polymorphic GAS and TAS sites in the U3 region, further suggest that a population different from classically activated macrophages can be infected by these viruses, opening new insights into lentiviruses with low or null pathogenic potential. This work was supported by Regione Piemonte, Ricerca Sanitaria Finalizzata 2009. |
| Databáze: | OpenAIRE |
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