Inhibition of vancomycin-resistant enterococcus by continuous-flow cultures of human stool microflora with and without anaerobic gas supplementation
| Autor: | Toni L. Poole, Michael E. Hume, Nicole J. Pultz, Curtis J. Donskey, Jennifer A. Hanrahan |
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| Rok vydání: | 2004 |
| Předmět: |
Culture model
Enterococcus faecium Colony Count Microbial medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Bacteria Anaerobic Feces Vancomycin Antibiosis Drug Resistance Bacterial medicine Humans Vancomycin-resistant Enterococcus Anaerobiosis biology Continuous flow Obligate anaerobe Vancomycin Resistance General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification DNA Fingerprinting Competitive exclusion Aerobiosis Anti-Bacterial Agents Enterococcus Room air distribution Electrophoresis Polyacrylamide Gel Anaerobic exercise |
| Zdroj: | Current microbiology. 48(5) |
| ISSN: | 0343-8651 |
| Popis: | A continuous-flow competitive exclusion (CFCE) culture model of human stool microflora was used to examine whether supplemental anaerobic gas is necessary for maintenance of anaerobes and inhibition of vancomycin-resistant Enterococcus (VRE). CFCE cultures of human stool microflora were maintained with supplemental nitrogen, without supplemental nitrogen, or with percolated room air. Cultures with or without supplemental nitrogen maintained9 log(10) CFU mL(-1) of obligate anaerobes and eliminated 10(6) CFU mL(-1) of VRE. When room air was percolated into the culture, anaerobes were detected ator =2 log(10) CFU mL(-1), and the same VRE inoculum was not eliminated ( P0.001). These data demonstrate that human stool CFCE cultures maintain high levels of obligate anaerobes and inhibit VRE without the addition of supplemental anaerobic gas. |
| Databáze: | OpenAIRE |
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