Structure, expression, chromosomal location and product of the gene encoding Adh2 in Petunia
| Autor: | Robert G. Gregerson, J N Strommer, P Dennis, L Cameron, M D McLean |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Molecular Sequence Data
Gene Expression Investigations Genes Plant Polymerase Chain Reaction Zea mays Petunia Molecular evolution Gene expression Genetics Gene family Genomic library Amino Acid Sequence Cloning Molecular Gene Peptide sequence Phylogeny Base Sequence biology Alcohol Dehydrogenase Nucleic acid sequence Chromosome Mapping DNA Plants biology.organism_classification Polymorphism Restriction Fragment Length |
| Zdroj: | Genetics. 133:999-1007 |
| ISSN: | 1943-2631 |
| Popis: | In most higher plants the genes encoding alcohol dehydrogenase comprise a small gene family, usually with two members. The Adh1 gene of Petunia has been cloned and analyzed, but a second identifiable gene was not recovered from any of three genomic libraries. We have therefore employed the polymerase chain reaction to obtain the major portion of a second Adh gene. From sequence, mapping and northern data we conclude this gene encodes ADH2, the major anaerobically inducible Adh gene of Petunia. The availability of both Adh1 and Adh2 from Petunia has permitted us to compare their structures and patterns of expression to those of the well-studied Adh genes of maize, of which one is highly expressed developmentally, while both are induced in response to hypoxia. Despite their evolutionary distance, evidenced by deduced amino acid sequence as well as taxonomic classification, the pairs of genes are regulated in strikingly similar ways in maize and Petunia. Our findings suggest a significant biological basis for the regulatory strategy employed by these distant species for differential expression of multiple Adh genes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|