Chilling-responsive DEMETER-LIKE DNA demethylase mediates in poplar bud break
| Autor: | Anne-Laure Le Gac, Isabel Allona, Christopher Dervinis, Daniel Conde, Matias Kirst, Pablo González-Melendi, Stéphane Maury, Mariano Perales |
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| Přispěvatelé: | Universidad Politécnica de Madrid (UPM), Laboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), Institut National de la Recherche Agronomique (INRA)-Université d'Orléans (UO), University of Florida [Gainesville] (UF), Genetics Institute, Emek Medical Center, COST Action, MINECO, US NSFPGRP, Consejo Social of the Universidad Politecnica de Madrid, Universidad Politecnica de Madrid, KBBE |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Plant Science DEMETER Genes Plant 01 natural sciences Transcriptome 03 medical and health sciences Annual growth cycle of grapevines bud break Gene Expression Regulation Plant winter dormancy Botany [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Plant Proteins DNA methylation biology Gene Expression Profiling fungi Gene Expression Regulation Developmental food and beverages 15. Life on land Apex (geometry) Cold Temperature DNA Demethylation Populus 030104 developmental biology DNA demethylation poplar Shoot biology.protein Demethylase Dormancy DNA demethylases Plant Shoots 010606 plant biology & botany |
| Zdroj: | Plant, Cell and Environment Plant, Cell and Environment, Wiley, 2017, 40 (10), pp.2236-2249. ⟨10.1111/pce.13019⟩ |
| ISSN: | 0140-7791 1365-3040 |
| Popis: | International audience; Annual dormancy-growth cycle is a developmental and physiological process essential for the survival of deciduous trees in temperate and boreal forests. Seasonal control of shoot growth in woody perennials requires specific genetic programmes responding to environmental signals. The environmental-controlled mechanisms that regulate the shift between winter dormancy and the growth-promoting genetic programmes are still unknown. Here, we show that dynamics in genomic DNA methylation levels are involved in the regulation of dormancy-growth cycle in poplar. The reactivation of growth in the apical shoot during bud break process in spring is preceded by a progressive reduction of genomic DNA methylation in apex tissue. The induction in apex tissue of a chilling-dependent poplar DEMETER-LIKE 10 (PtaDML10) DNA demethylase precedes shoot growth reactivation. Transgenic poplars showing downregulation of PtaDML8/10 caused delayed bud break. Genome-wide transcriptome and methylome analysis and data mining revealed that the gene targets of DEMETER-LIKE-dependent DNA demethylation are genetically associated with bud break. These data point to a chilling-dependent DEMETER-like DNA demethylase mechanisms being involved in the shift from winter dormancy to a condition that precedes shoot apical vegetative growth in poplar. |
| Databáze: | OpenAIRE |
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