The circular RNA hsa_circ_0007623 acts as a sponge of microRNA-297 and promotes cardiac repair
| Autor: | Jiandong Chen, Qian Zhang, Peng Yu, Manlu Fan, Weixin Sun, Le Shen, Jie Han, Songyi Cheng, Haowen Zhang, Xiaohu Chen, Huaqin Tong |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Vascular Endothelial Growth Factor A 0301 basic medicine Angiogenesis Myocardial Ischemia Biophysics Neovascularization Physiologic Biochemistry Umbilical vein Pathogenesis 03 medical and health sciences 0302 clinical medicine Cell Movement Circular RNA microRNA Human Umbilical Vein Endothelial Cells Animals Humans Regeneration Gene silencing Molecular Biology Cell Proliferation Mice Inbred BALB C Base Sequence Cell growth Chemistry Myocardium Isoproterenol RNA Circular Cell Biology Cell biology MicroRNAs Vascular endothelial growth factor A 030104 developmental biology Gene Expression Regulation Gene Knockdown Techniques 030220 oncology & carcinogenesis Cardiomyopathies Signal Transduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 523:993-1000 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2019.12.116 |
| Popis: | Circular RNAs (circRNAs) are a kind of closed loop endogenous non-coding RNAs have attracted increasing interest in recent years. However, the mechanism of circRNAs in the pathogenesis of multiple cardiovascular diseases, particularly myocardial ischemia, is rarely reported. In the present study, we examined a circular RNA, hsa_circ_0007623, which is highly expressed in hypoxia-induced human umbilical vein endothelial cells (HUVECs) and can act as a sponge for miR-297, which is involved in cardiac repair after acute myocardial ischemia. In hypoxia-stimulated HUVECs, the inhibition of hsa_circ_0007623 expression was found to reduce cell proliferation, migration, and angiogenesis. Further in vivo experiments confirmed the cardioprotective effect of hsa_circ_0007623 expression in isoproterenol-induced acute ischemia mice. Bioinformatics analysis predicted hsa_circ_0007623, sponge miR-297 and miR-297 directly target VEGFA, which was validated by dual-luciferase assay. Subsequently, functional experiments revealed hsa_circ_0007623 silencing could up-regulate miR-297 and down-regulate VEGFA expression, and reduce cell proliferation, migration, and angiogenesis. We concluded that hsa_circ_0007623 can bind to miR-297, promote cardiac repair after acute myocardial ischemia, and protect cardiac function. |
| Databáze: | OpenAIRE |
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