Serum phospholipase A2 enzyme activity and immunoreactivity in a prospective analysis of patients with septic shock
| Autor: | Peter Vadas, Kieran F. Scott, Eva Stefanski, G. Smith, R. Singh, B.D. Schouten, I. A. Rajkovic, Waldemar Pruzanski |
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| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.medical_specialty Circulatory collapse Phospholipid Enzyme-Linked Immunosorbent Assay Phospholipases A General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Phospholipase A2 Internal medicine medicine Humans Prospective Studies General Pharmacology Toxicology and Pharmaceutics chemistry.chemical_classification biology Septic shock Antibodies Monoclonal General Medicine medicine.disease Shock Septic Enzyme assay Phospholipases A2 Enzyme Endocrinology chemistry Shock (circulatory) Immunology biology.protein Female lipids (amino acids peptides and proteins) Antibody medicine.symptom |
| Zdroj: | Life Sciences. 50:807-811 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/0024-3205(92)90186-s |
| Popis: | Massive elevations of serum phospholipase A2 activity have been documented in patients with septic shock. Serum PLA2 activity correlated to the degree and duration of circulatory collapse, while purified native PLA2 reproduced hypotension in experimental animals. In a prospective study of patients with septic shock, we have determined the relationship of PLA2 enzyme activity to PLA2 immunoreactivity using radiolabelled E. coli phospholipid substrate and an ELISA specific for group II human nonpancreatic PLA2. In all patients, there was a clear concordance of the two assays. Maximal PLA2 concentration was increased a mean of 554-fold over normal levels. We found no evidence to support the presence of activating or inhibitory proteins. These data confirm that the observed increase in serum PLA2 activity in septic shock is due to intravascular release of group II nonpancreatic PLA2. |
| Databáze: | OpenAIRE |
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