The Type III Effectome of the Symbiotic Bradyrhizobium vignae Strain ORS3257
| Autor: | Alicia Camuel, Peter Mergaert, David Cornu, Albin Teulet, Dany Severac, Nicolas Busset, Eric Giraud, Djamel Gully, Joël Fardoux |
|---|---|
| Přispěvatelé: | Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire des symbioses tropicales et méditerranéennes (UMR LSTM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE20-0013,SymEffectors,Système de sécrétion de type 3 pour la symbiose fixatrice d'azote(2016), ANR-20-CE20-0012,ET-Nod,Effecteurs déclenchant la nodulation chez les légumineuses(2020), Gully, Djamel [0000-0003-1695-5744], Camuel, Alicia [0000-0003-3912-9613], Mergaert, Peter [0000-0002-5919-7317], Apollo - University of Cambridge Repository, Astruc, Suzette, Système de sécrétion de type 3 pour la symbiose fixatrice d'azote - - SymEffectors2016 - ANR-16-CE20-0013 - AAPG2016 - VALID, Effecteurs déclenchant la nodulation chez les légumineuses - - ET-Nod2020 - ANR-20-CE20-0012 - AAPG2020 - VALID |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
legume symbiosis
In silico proteome Mutant Computational biology Biochemistry Bradyrhizobium Microbiology Type three secretion system Nod factor Transcriptome 03 medical and health sciences nod factor [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Type III Secretion Systems nodulation [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Symbiosis Molecular Biology 030304 developmental biology 0303 health sciences biology 030306 microbiology Effector food and beverages biology.organism_classification QR1-502 type III secretion system effector Proteome transcriptome |
| Zdroj: | Biomolecules Biomolecules, 2021, 11 (11), ⟨10.3390/biom11111592⟩ Volume 11 Issue 11 Biomolecules, Vol 11, Iss 1592, p 1592 (2021) |
| ISSN: | 2218-273X |
| Popis: | Many Bradyrhizobium strains are able to establish a Nod factor-independent symbiosis with the leguminous plant Aeschynomene indica by the use of a type III secretion system (T3SS). Recently, an important advance in the understanding of the molecular factors supporting this symbiosis has been achieved by the in silico identification and functional characterization of 27 putative T3SS effectors (T3Es) of Bradyrhizobium vignae ORS3257. In the present study, we experimentally extend this catalog of T3Es by using a multi-omics approach. Transcriptome analysis under non-inducing and inducing conditions in the ORS3257 wild-type strain and the ttsI mutant revealed that the expression of 18 out of the 27 putative effectors previously identified, is under the control of TtsI, the global transcriptional regulator of T3SS and T3Es. Quantitative shotgun proteome analysis of culture supernatant in the wild type and T3SS mutant strains confirmed that 15 of the previously determined candidate T3Es are secreted by the T3SS. Moreover, the combined approaches identified nine additional putative T3Es and one of them was experimentally validated as a novel effector. Our study underscores the power of combined proteome and transcriptome analyses to complement in silico predictions and produce nearly complete effector catalogs. The establishment of the ORS3257 effectome will form the basis for a full appraisal of the symbiotic properties of this strain during its interaction with various host legumes via different processes. |
| Databáze: | OpenAIRE |
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