Characteristics of perforin expressing lymphocytes within the first trimester decidua of human pregnancy
| Autor: | Lea Gudelj, Eckhard R. Podack, Daniel Rukavina, Herman Haller, Gordana Rubeša |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Pore Forming Cytotoxic Proteins
medicine.medical_specialty Lymphocyte CD3 CD8 Antigens T-Lymphocytes Immunology Decidua Parietalis Perforin Decidua Natural-killer-cells Metrial gland-cells Flow-cytometry chemical and pharmacologic phenomena CD8-Positive T-Lymphocytes Immunophenotyping Pregnancy Internal medicine medicine Immunology and Allergy Cytotoxic T cell Humans Membrane Glycoproteins biology Staining and Labeling Obstetrics and Gynecology hemic and immune systems Molecular biology CD56 Antigen Killer Cells Natural Pregnancy Trimester First medicine.anatomical_structure Endocrinology Reproductive Medicine biology.protein Female Decidua Basalis CD8 Biomarkers |
| Zdroj: | American journal of reproductive immunology (New York, N.Y. : 1989). 33(5) |
| ISSN: | 1046-7408 |
| Popis: | PROBLEM: The number of perforin (P)-positive cells in decidua of pregnancy is larger than that observed in any other pathological condition. The aim was to investigate the distribution and the phenotype of P + cells. METHOD: Decidual tissue was obtained from the first trimester vaginal termination of pregnancy. Tissue distribution of P + cells was analyzed by immunohistochemistry. The method for simultaneous measurement of P and cell surface is presented. RESULTS: There is no difference in number and distribution of P + cells between decidua basalis (DB) and decidua parietalis (DP). The percentage of P + decidual lymphocytes (DL) is two times higher than in peripheral blood lymphocytes (PBL) (55% vs. 27%), and the prevalent phenotype is CD3 - CD4 - CD8 - CD2 + (95%) CD11c + (68%) and CD56 + (82%). CD56 bright+ DL are also P bright+ and this is the largest DL subpopulation (42.4% DL). Two different subpopulations of CD8 + DL exist: 1) CD8 bright+ , which are CD3 + CD56 - P - and 2) CD8 dim+ , which are CD3 - CD56 + P + . CONCLUSION: P expressing DL are prevalently nonclassical NK cells (CD16 - ) with low cytolytic activity but fully equipped with potent cytolytic machinery (P bright+ ). There are no classical cytotoxic lymphocytes (CTL) (CD3 + CD8 + P + ) in the decidua, and all CD8 + P + cells are CD3 - CD56 + . The number of P + cells is even higher in DP in the vicinity of noninvasvie trophoblast, than in DB |
| Databáze: | OpenAIRE |
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