Molecular Characterization of Quinolone-Resistant Neisseria gonorrhoeae Isolates from Brazil ▿
| Autor: | Marcia Giambiagi-deMarval, Maria de Fátima Ferreira, Ivano de Filippis, Felipe P.G. Neves, Maysa M. Clementino, Lúcia M. Teixeira, Aline A. Uehara, Efigênia L. T. Amorin, Tatiana C. A. Pinto, Sergio Eduardo Longo Fracalanzza, Claudia Ferreira de Andrade |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Microbiology (medical)
DNA Topoisomerase IV DNA Bacterial Genotype medicine.drug_class Antibiotics Mutation Missense Drug resistance Microbial Sensitivity Tests Biology Quinolones medicine.disease_cause Microbiology Gonorrhea Antibiotic resistance Drug Resistance Bacterial medicine Cluster Analysis Humans Molecular Epidemiology Molecular epidemiology Bacteriology Quinolone Virology Neisseria gonorrhoeae Anti-Bacterial Agents Electrophoresis Gel Pulsed-Field Ciprofloxacin Molecular Typing Amino Acid Substitution DNA Gyrase Brazil medicine.drug |
| Popis: | Despite the rapid spread of antibiotic resistance among gonococci worldwide, limited reports are available from Brazilian locations. In the present study, 25 quinolone-resistant Neisseria gonorrhoeae (QRNG) strains isolated in Rio de Janeiro, Brazil, were characterized by phenotypic and molecular methods, including analysis of mutations in the gyrA and parC genes. They represented 16.5% of the N. gonorrhoeae isolates obtained during a survey performed from 2006 to 2010. A trend for increasing resistance to ciprofloxacin was observed in the period investigated. The most prevalent pattern of mutation observed among QRNG isolates, Ser-91 to Phe and Asp-95 to Gly in gyrA and Ser-87 to Arg in parC , was detected in 40% of the isolates exhibiting MICs ranging from 4 to >32 μg/ml. Rare types of mutations were found in the gyrA gene (Gln-102 to His [12%] and Asp-95 to Tyr [4%]) and in the parC gene (Ser-88 to Thr [4%]). The genetic relationship of the QRNG isolates, evaluated by pulsed-field gel electrophoresis, suggested that the increase in the frequencies of the QRNG isolates in Rio de Janeiro, Brazil, may have arisen as a result of simultaneous spread of two clonal groups. The results also indicate that fluoroquinolones may no longer be used as first line antibiotics for the treatment of gonorrhea in Rio de Janeiro, and that programs for antimicrobial susceptibility surveillance of N. gonorrhoeae should also be implemented in other regions of Brazil. |
| Databáze: | OpenAIRE |
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