An inactivated influenza D virus vaccine partially protects cattle from respiratory disease caused by homologous challenge
| Autor: | Kelly Lechtenberg, Lucas Huntimer, Shollie M. Falkenberg, Jamie Henningson, Tom Halbur, Ben M. Hause |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences Bovine Respiratory Disease Complex Cattle Diseases Bovine respiratory disease Pathogenesis Biology Antibodies Viral Microbiology Article Virus 0403 veterinary science 03 medical and health sciences Tracheitis Orthomyxoviridae Infections medicine Animals Viral shedding Antigens Viral Respiratory disease Hemagglutination assay General Veterinary medicine.diagnostic_test Vaccination Viral Vaccines Bovine 04 agricultural and veterinary sciences General Medicine Viral Load medicine.disease Virology Influenza Virus Shedding 030104 developmental biology Bronchoalveolar lavage Vaccines Inactivated Inactivated vaccine Immunology Cattle Thogotovirus Vaccine |
| Zdroj: | Veterinary Microbiology |
| ISSN: | 0378-1135 |
| Popis: | Highlights • The emerging influenza D virus (IDV) caused respiratory disease in calves. • A homologous inactivated vaccine was immunogenic and partially protective. • IDV titers in vaccinated calves were significantly reduced compared to controls. • Fewer vaccinates were positive for IDV by immunohistochemistry. • These results demonstrate an etiologic role for IDV in bovine respiratory disease. Originally isolated from swine, the proposed influenza D virus has since been shown to be common in cattle. Inoculation of IDV to naïve calves resulted in mild respiratory disease histologically characterized by tracheitis. As several studies have associated the presence of IDV with acute bovine respiratory disease (BRD), we sought to investigate the efficacy of an inactivated IDV vaccine. Vaccinated calves seroconverted with hemagglutination inhibition titers 137–169 following two doses. Non-vaccinated calves challenged with a homologous virus exhibited signs of mild respiratory disease from days four to ten post challenge which was significantly different than negative controls at days five and nine post challenge. Peak viral shedding of approximately 5 TCID50/mL was measured in nasal and tracheal swabs and bronchoalveolar lavage fluids four to six days post challenge. Viral titers were significantly (P |
| Databáze: | OpenAIRE |
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