Targeting Anthrax Toxin Receptor 2 Ameliorates Endometriosis Progression
| Autor: | Hsiu Chi Lee, Yi Han Huang, Shaw Jenq Tsai, Shih Chieh Lin, Meng Hsing Wu, Pei Ling Hsu, Ching Ting Hsu, Wan Ning Li |
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| Rok vydání: | 2019 |
| Předmět: |
endometriosis
0301 basic medicine Stromal cell Receptors Peptide Angiogenesis Endometriosis Medicine (miscellaneous) 03 medical and health sciences Histone H3 0302 clinical medicine Downregulation and upregulation Animals Humans Medicine EZH2 Pharmacology Toxicology and Pharmaceutics (miscellaneous) Cells Cultured YAP1 030219 obstetrics & reproductive medicine hypoxia business.industry Anthrax toxin receptor 2 Gene Expression Profiling cell adhesion medicine.disease ANTXR2 Mice Inbred C57BL Disease Models Animal 030104 developmental biology Cancer research Female business Research Paper |
| Zdroj: | Theranostics |
| ISSN: | 1838-7640 |
| DOI: | 10.7150/thno.30655 |
| Popis: | Rationale: Endometriosis is a highly prevalent gynecological disease in women of reproductive age that markedly reduces life quality and fertility. Unfortunately, there is no cure for this disease, which highlights that more efforts are needed to investigate the underlying mechanism for designing novel therapeutic regimens. This study aims to investigate druggable membrane receptors distinctively expressed in endometriotic cells. Methods: Bioinformatic analysis of public databases was employed to identify potential druggable candidates. Normal endometrial tissues and ectopic endometriotic lesions were obtained for the determination of target genes. Primary endometrial and endometriotic stromal cells as well as two different mouse models of endometriosis were used to characterize molecular mechanisms and therapeutic outcomes of endometriosis, respectively. Results: Anthrax toxin receptor 2 (ANTXR2) mRNA and protein are upregulated in the endometriotic specimens. Elevation of ANTXR2 promotes endometriotic cell adhesion, proliferation, and angiogenesis. Furthermore, hypoxia is the driving force for ANTXR2 upregulation via altering histone modification of ANTXR2 promoter by reducing the repressive mark, histone H3 lysine 27 (H3K27) trimethylation, and increasing the active mark, H3K4 trimethylation. Activation of ANTXR2 signaling leads to increased Yes-associated protein 1 (YAP1) nuclear translocation and transcriptional activity, which contributes to numerous pathological processes of endometriosis. Pharmacological blocking of ANTXR2 signaling not only prevents endometriotic lesion development but also causes the regression of established lesion. Conclusion: Taken together, we have identified a novel target that contributes to the disease pathogenesis of endometriosis and provided a potential therapeutic regimen to treat it. |
| Databáze: | OpenAIRE |
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