The [PSI+] prion of yeast: A problem of inheritance
| Autor: | Mick F. Tuite, Brian S. Cox |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Prions animal diseases Molecular Sequence Data Saccharomyces cerevisiae Models Biological General Biochemistry Genetics and Molecular Biology Amino Acid Sequence Molecular Biology Genetics biology A protein Conclusive evidence biology.organism_classification Yeast nervous system diseases Fungal prion Phenotype Chaperone (protein) Mutation Genetic element biology.protein Cell Division Molecular Chaperones Peptide Termination Factors |
| Zdroj: | Methods. 39:9-22 |
| ISSN: | 1046-2023 |
| DOI: | 10.1016/j.ymeth.2006.04.001 |
| Popis: | The [PSI(+)] prion of the yeast Saccharomyces cerevisiae was first identified by Brian Cox some 40 years ago as a non-Mendelian genetic element that modulated the efficiency of nonsense suppression. Following the suggestion by Reed Wickner in 1994 that such elements might be accounted for by invoking a prion-based model, it was subsequently established that the [PSI(+)] determinant was the prion form of the Sup35p protein. In this article, we review how a combination of classical genetic approaches and modern molecular and biochemical methods has provided conclusive evidence of the prion basis of the [PSI(+)] determinant. In so doing we have tried to provide a historical context, but also describe the results of more recent experiments aimed at elucidating the mechanism by which the [PSI(+)] (and other yeast prions) are efficiently propagated in dividing cells. While understanding of the [PSI(+)] prion and its mode of propagation has, and will continue to have, an impact on mammalian prion biology nevertheless the very existence of a protein-based mechanism that can have a beneficial impact on a cell's fitness provides equally sound justification to fully explore yeast prions. |
| Databáze: | OpenAIRE |
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