Molecular detection of the reptile-associated Borrelia group in Amblyomma dissimile, Mexico
| Autor: | Ingeborg Becker, Estefania Grostieta, Sokani Sánchez-Montes, Leticia M. Ochoa-Ochoa, Pablo Colunga-Salas |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Ixodidae 030231 tropical medicine Zoology Tick DNA sequencing Amphibians 03 medical and health sciences 0302 clinical medicine Amblyomma Borrelia RNA Ribosomal 16S Animals Amblyomma dissimile Pathology Molecular Mexico Ecology Evolution Behavior and Systematics Phylogeny Borrelia sp General Veterinary biology Reptiles 030108 mycology & parasitology bacterial infections and mycoses biology.organism_classification 16S ribosomal RNA Bufonidae Tick Infestations Genes Bacterial Insect Science Vector (epidemiology) Rhinella Parasitology Arachnid Vectors |
| Zdroj: | Medical and veterinary entomologyReferences. 35(2) |
| ISSN: | 1365-2915 |
| Popis: | Recently, the first record of Borrelia associated with reptiles in Mexico was published; however, no studies have been done to assess the role of Mexican ticks as potential vectors of this Borrelia group. Amblyomma dissimile is a hard tick mainly associated with amphibians and reptiles in this country. The aim of this study was to evaluate the presence of Borrelia in A. dissimile from Mexico. We collected 60 A. dissimile individuals attached to 16 Rhinella horribilis. DNA was extracted and all specimens were screened individually for Borrelia by amplification of a fragment of the 16S rDNA and an additional fragment of the flagellin gene. Five ticks were positive for Borrelia, DNA sequences corresponded to Borrelia sp. and group with sequences of the reptile-associated Borrelia group. This is the first report of Borrelia in A. dissimile and the second report of the reptile-associated Borrelia group in Mexico. This study also highlights the importance of this tick species as potential vector of this group. |
| Databáze: | OpenAIRE |
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