Lactate Metabolism in Breast Cancer Microenvironment: Contribution Focused on Associated Adipose Tissue and Obesity

Autor: Biljana Srdic Galic, Marija Aleksic, Aleksandra Jankovic, Aleksandra Korac, Bato Korac, Mirjana Udicki, Andjelika Kalezic
Jazyk: angličtina
Rok vydání: 2020
Předmět:
obesity
Receptor
ErbB-2
Adipose tissue
lcsh:Chemistry
chemistry.chemical_compound
0302 clinical medicine
Lactate oxidation
Tumor Microenvironment
Glycolysis
lcsh:QH301-705.5
Spectroscopy
0303 health sciences
Chemistry
Carcinoma
Ductal
Breast
General Medicine
Prognosis
3. Good health
Computer Science Applications
Isoenzymes
Adipose Tissue
Receptors
Estrogen
030220 oncology & carcinogenesis
Female
Receptors
Progesterone
Adult
Breast Neoplasms
Article
Catalysis
Inorganic Chemistry
03 medical and health sciences
Breast cancer
premenopausal breast cancer
Lactate dehydrogenase
medicine
Humans
cancer-associated adipose tissue
Neoplasm Invasiveness
Lactic Acid
Physical and Theoretical Chemistry
Molecular Biology
030304 developmental biology
lactate
L-Lactate Dehydrogenase
Organic Chemistry
Cancer
lactate dehydrogenase
medicine.disease
lcsh:Biology (General)
lcsh:QD1-999
Cancer cell
Cancer research
Homeostasis
Follow-Up Studies
Zdroj: International Journal of Molecular Sciences
Volume 21
Issue 24
International Journal of Molecular Sciences, Vol 21, Iss 9676, p 9676 (2020)
ISSN: 1422-0067
DOI: 10.3390/ijms21249676
Popis: Metabolic reprogramming that favors high glycolytic flux with lactate production in normoxia is among cancer hallmarks. Lactate is an essential oncometabolite regulating cellular redox homeostasis, energy substrate partitioning, and intracellular signaling. Moreover, malignant phenotype&rsquo
s chief characteristics are dependent on the interaction between cancer cells and their microenvironment. In breast cancer, mammary adipocytes represent an essential cellular component of the tumor milieu. We analyzed lactate concentration, lactate dehydrogenase (LDH) activity, and isozyme pattern, and LDHA/LDHB protein expression and tissue localization in paired biopsies of breast cancer tissue and cancer-associated adipose tissue in normal-weight and overweight/obese premenopausal women, compared to benign breast tumor tissue and adipose tissue in normal-weight and overweight/obese premenopausal women. We show that higher lactate concentration in cancer tissue is concomitant with a shift in isozyme pattern towards the &ldquo
muscle-type&rdquo
LDH and corresponding LDHA and LDHB protein expression changes. In contrast, significantly higher LDH activity in cancer-associated adipose tissue seems to be directed towards lactate oxidation. Moreover, localization patterns of LDH isoforms varied substantially across different areas of breast cancer tissue. Invasive front of the tumor showed cell-specific protein localization of LDHA in breast cancer cells and LDHB in cancer-associated adipocytes. The results suggest a specific, lactate-centric relationship between cancer tissue and cancer-associated adipose tissue and indicate how cancer-adipose tissue cross-talk may be influenced by obesity in premenopausal women.
Databáze: OpenAIRE
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