Different conformational forms of Escherichia coli and rat liver 5S rRNA revealed by Pb(II)-induced hydrolysis
| Autor: | Volker A. Erdmann, Siegfried Lorenz, Jerzy Ciesiołka |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Stereochemistry
Base pair Cations Divalent Molecular Sequence Data Biology medicine.disease_cause Biochemistry 5S ribosomal RNA medicine Escherichia coli Animals Nucleotide chemistry.chemical_classification Base Sequence Hydrolysis RNA Ribosomal 5S RNA Ribosomal RNA Rats chemistry Lead Helix Nucleic Acid Conformation Electrophoresis Polyacrylamide Gel Alpha helix |
| Zdroj: | European journal of biochemistry. 204(2) |
| ISSN: | 0014-2956 |
| Popis: | Different stable forms of Escherichia coli and rat liver 5S rRNA have been probed by Pb(II)-induced hydrolysis. In the native A forms of 5S rRNA, Pb2+ reveal single-stranded RNA stretches and regions of increased conformational flexibility or distorted by the presence of bulged nucleotides. Hydrolysis of urea/EDTA-treated E. coli 5S rRNA (B form) shows the presence of two strong helical domains; helix A retained from the A form and a helix composed of RNA regions G33–C42 and G79–C88. Other RNA regions resistant to hydrolysis may be involved in alternative base pairing, causing conformational heterogeneity of that form. Pb(II)-induced hydrolysis distinguishes two different forms of rat liver 5S rRNA; the native A form and the form obtained by renaturation of 5S rRNA in the presence of EDTA. Pb(II)-hydrolysis data suggest that both forms are highly structured. In the latter form, the orientation of the bulged C66 is changed with respect to helix B. At the same time, a new helical segment is possibly formed, composed of nucleotides from helix C and loop c on one side and from helix E and loop d' on the other. |
| Databáze: | OpenAIRE |
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