Changes in plasma miR-9, miR-16, miR-205 and miR-486 levels after non-small cell lung cancer resection
| Autor: | Maciej Turski, Magdalena Chechlinska, Maria Sromek, Maciej Glogowski, Robert Wlodarczyk, Mariusz Kulinczak, Joanna Owczarek, Lukasz Szafron, Klara Zakrzewska, Lukasz Talarek, Jan Konrad Siwicki, Piotr Wisniewski |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Oncology Adult Male Cancer Research medicine.medical_specialty Lung Neoplasms Disease Resection Diagnosis Differential 03 medical and health sciences 0302 clinical medicine Internal medicine Carcinoma Non-Small-Cell Lung microRNA medicine Biomarkers Tumor Humans Lung cancer Aged Aged 80 and over business.industry Cancer General Medicine Middle Aged medicine.disease Gene Expression Regulation Neoplastic Circulating MicroRNA MicroRNAs 030104 developmental biology Treatment Outcome 030220 oncology & carcinogenesis Molecular Medicine Adenocarcinoma Female Non small cell business |
| Zdroj: | Cellular oncology (Dordrecht). 40(5) |
| ISSN: | 2211-3436 |
| Popis: | The majority of non-small cell lung cancer (NSCLC) patients presents with an advanced-stage disease and, consequently, exhibits a poor overall survival rate. We aimed to assess changes in plasma miR-9, miR-16, miR-205 and miR-486 levels and their potential as biomarkers for the diagnosis and monitoring of NSCLC patients. Plasma was collected from 50 healthy donors and from NSCLC patients before surgery (n = 61), 1 month after surgery (n = 37) and 1 year after surgery (n = 14). microRNA levels were quantified using qRT-PCR. We found in NSCLC patients before treatment, both with squamous cell carcinoma (SQCC) and adenocarcinoma (ADC), significantly higher plasma miR-16 and miR-486 levels than in healthy individuals. Pre-treatment miR-205 concentrations were found to be significantly higher in SQCC than in ADC patients, and only SQCC patients presented significantly higher circulating miR-205 levels than healthy donors. SQCC plasma miR-9 levels were not different from normal control levels, but in ADC they were found to be significantly decreased. A combination of plasma miR-16, miR-205 and miR-486 measurements was found to discriminate NSCLC patients from healthy persons, with a specificity of 95% and a sensitivity of 80%. Following tumor resection, we found that the miR-9 and miR-205 levels significantly decreased, even below the normal level, whereas the increased miR-486 level persisted up to one year after surgery, and the miR-16 level decreased to normal. After tumor resection, none of the miR levels tested was found to relate to recurrence. Our data indicate that miR-9, miR-16, miR-205 and miR-486 may serve as NSCLC biomarkers. The observed cancer-related pre- and post-operative changes in their plasma levels may not only reflect the presence of a primary cancer, but also of a systemic response to cancer. |
| Databáze: | OpenAIRE |
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