Human skin explant model for the investigation of topical therapeutics
| Autor: | Marc B. Brown, Adrian C. Williams, Jessica Neil |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Keratinocytes
0301 basic medicine Administration Topical Skin Absorption Human skin In Vitro Techniques Bioinformatics Models Biological Article 03 medical and health sciences Organ Culture Techniques 0302 clinical medicine In vivo Humans Medicine Animal testing Barrier function Skin Multidisciplinary business.industry Biological techniques Proteins Metabolic stability In vitro 030104 developmental biology 030220 oncology & carcinogenesis business Ex vivo Biotechnology Explant culture |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The development of in vitro and ex vivo models to mimic human illness is important not only for scientific understanding and investigating therapeutic approaches but also to mitigate animal testing and bridge the inter-species translational gap. While in vitro models can facilitate high-throughput and cost-efficient evaluation of novel therapeutics, more complex ex vivo systems can better predict both desirable and adverse in vivo effects. Here we describe an ex vivo cultured human skin explant model in which we have characterized pathological tissue integrity, barrier function and metabolic stability over time. Our findings suggest that human skin can be successfully cultured for pharmacodynamic use up to and beyond 9 days without any adverse physiological consequence. |
| Databáze: | OpenAIRE |
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