Characterisation of blood-derived exosomal hTERT mRNA secretion in cancer patients: a potential pan-cancer marker
| Autor: | Orit Uziel, Ofir Wolach, Jardena Nordenberg, Meir Lahav, Ester Rabizadeh, Yonatan Edel, Anna Gutkin, Einat Beery, Hadar Goldvaser |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research exosomes Biology telomerase Disease course 03 medical and health sciences Young Adult 0302 clinical medicine Neoplasms medicine Biomarkers Tumor Humans Secretion RNA Messenger neoplasms Molecular Diagnostics Aged Neoplasm Staging Aged 80 and over Messenger RNA Pan cancer RNA Cancer Middle Aged medicine.disease Molecular biology enzymes and coenzymes (carbohydrates) Htert mrna 030104 developmental biology Oncology 030220 oncology & carcinogenesis Case-Control Studies embryonic structures Feasibility Studies Neoplasm staging Female biological phenomena cell phenomena and immunity Neoplasm Grading hTERT |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 |
| Popis: | Background: Telomerase (human telomerase reverse transcriptase (hTERT)) is considered a hallmark of cancer. The aim of our study was to evaluate the feasibility of the detection of hTERT transcripts in serum as a ‘pan-cancer’ diagnostic method. Methods: Human telomerase reverse transcriptase mRNA levels were determined in serum and serum-derived exosomes from 133 patients with different malignancies and 45 healthy controls. In four patients hTERT mRNA levels were measured in different clinical stages. Results: Human telomerase reverse transcriptase transcript was absent in all controls and was variably detected in 67.5% of patients with all cancer types. A correlation between hTERT transcript levels and the clinical course was found in several cases. Conclusions: Human telomerase reverse transcriptase mRNA levels may reflect the tumour burden and the clinical status of the patient. In patients with detectable levels, this assay may potentially serve as a diagnostic and follow-up ‘pan-cancer’ marker. Owing to the large variety of patients and small sample size in each diagnosis, the statistical power is limited and will be explored further in larger groups. |
| Databáze: | OpenAIRE |
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