Embryo size regulates the timing and mechanism of pluripotent tissue morphogenesis
| Autor: | Viviane Souza Rosa, Lorenzo Carlo Orietti, Marta N. Shahbazi, Christos Kyprianou, Francesco Antonica, Magdalena Zernicka-Goetz, Henrique Marques-Souza, William Mansfield |
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| Přispěvatelé: | Zernicka-Goetz, Magdalena [0000-0002-7004-2471], Apollo - University of Cambridge Repository |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
animal structures Time Factors Morphogenesis embryo morphogenesis Cell fate determination Biology Biochemistry Exocytosis Article lumenogenesis 03 medical and health sciences 0302 clinical medicine epithelial tissue Embryonic morphogenesis mouse embryogenesis regulative development apoptosis size regulation embryonic stem cells implantation Genetics medicine Animals Amnion Cell Aggregation Basement membrane Embryogenesis Embryo Cell Biology Organ Size Embryo Mammalian Embryonic stem cell Cell biology Mice Inbred C57BL 030104 developmental biology medicine.anatomical_structure embryonic structures Mice Inbred CBA Female 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cell Reports |
| DOI: | 10.17863/cam.57283 |
| Popis: | Summary Mammalian embryogenesis is a paradigm of regulative development as mouse embryos show plasticity in the regulation of cell fate, cell number, and tissue morphogenesis. However, the mechanisms behind embryo plasticity remain largely unknown. Here, we determine how mouse embryos respond to an increase in cell numbers to regulate the timing and mechanism of embryonic morphogenesis, leading to the formation of the pro-amniotic cavity. Using embryos and embryonic stem cell aggregates of different size, we show that while pro-amniotic cavity formation in normal-sized embryos is achieved through basement membrane-induced polarization and exocytosis, cavity formation of increased-size embryos is delayed and achieved through apoptosis of cells that lack contact with the basement membrane. Importantly, blocking apoptosis, both genetically and pharmacologically, alters pro-amniotic cavity formation but does not affect size regulation in enlarged embryos. We conclude that the regulation of embryonic size and morphogenesis, albeit concomitant, have distinct molecular underpinnings. Graphical abstract Highlights • Increased-size embryos present a delay in embryonic morphogenesis • Embryo size determines the mechanism of pro-amniotic cavity formation • Apoptosis is required for the formation of a single cavity in enlarged embryos • Cavity formation and size regulation have distinct molecular underpinnings In this article Shahbazi, Zernicka-Goetz, and colleagues explore how increasing embryo size affects the mechanism and timing of embryonic morphogenesis. Enlarged embryos present a delay in pro-amniotic cavity formation, an essential morphogenetic event that happens concomitantly with the regulation of embryo size. However, whereas pro-amniotic cavity formation requires apoptosis of embryonic cells lacking basement membrane contact, size regulation occurs independently of cell death. |
| Databáze: | OpenAIRE |
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