Novel nucleoside triphosphate analogs for the enzymatic labeling of nucleic acids

Autor:	Glenn H. McGall, Anthony D. Barone, Philip R. Buzby, Chuan Chen, James Joseph Dimeo, K. Rafii
Rok vydání:	2001
Předmět:	chemistry.chemical_classification Nucleotides Biotin Nucleic Acid Hybridization General Medicine DNA-Directed RNA Polymerases Fluoresceins Biochemistry chemistry.chemical_compound Viral Proteins Enzyme chemistry Terminal deoxynucleotidyl transferase DNA Nucleotidylexotransferase Genetics Nucleoside triphosphate Nucleic acid medicine Molecular Medicine T7 RNA polymerase Nucleotide DNA Probes medicine.drug
Zdroj:	Nucleosides, nucleotidesnucleic acids. 20(4-7)
ISSN:	1525-7770
Popis:	We have evaluated several novel nucleotide analogs suitable for enzymatic labeling of nucleic acid targets for a variety of array-based assays. Two new reagents in particular, a C4-labeled 1-(2',3'-dideoxy-beta-D-ribofuranosyl) imidazole-4-carboxamide 5'-triphosphate 5 and an N1-labeled 5-(beta-D-ribofuranosyl)-2,4(1H,3H)-pyrimidinedione 5'-triphosphate 3, were found to be excellent substrates for labeling with terminal deoxynucleotidyl transferase and T7 RNA polymerase, respectively.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::be6f6275fdb4726fa7ff8b2c5243f5ec https://pubmed.ncbi.nlm.nih.gov/11562974 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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