Phosphorylation Time-Course Study of the Response during Adenovirus Type 2 Infection
| Autor: | Hongxing Zhao, Ulf Pettersson, Alberto Valdés, Sara Bergström Lind |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Proteomics
Proteome viruses GTPase macromolecular substances Biochemistry adenovirus type 2 infection Analytical Chemistry Adenovirus Infections Human 03 medical and health sciences human foetal lung fibroblasts (IMR-90) Analytisk kemi Humans Protein kinase A signaling Phosphorylation Molecular Biology 030304 developmental biology mass spectrometry 0303 health sciences Cyclin-dependent kinase 1 Chemistry Kinase 030302 biochemistry & molecular biology Phosphoproteomics phosphoproteomics Actin cytoskeleton Phosphoproteins Cell biology Signal transduction stable isotope labeling with amino acids in cell culture (SILAC) Protein Processing Post-Translational Signal Transduction |
| Popis: | PTMs such as phosphorylations are usually involved in signal transduction pathways. To investigate the temporal dynamics of phosphoproteome changes upon viral infection, a model system of IMR-90 cells infected with human adenovirus type 2 (Ad2) is used in a time-course quantitative analysis combining titanium dioxide (TiO2 ) particle enrichment and SILAC-MS. Quantitative data from 1552 phosphorylated sites clustered the highly altered phosphorylated sites to the signaling by rho family GTPases, the actin cytoskeleton signaling, and the cAMP-dependent protein kinase A signaling pathways. Their activation is especially pronounced at early time post-infection. Changes of several phosphorylated sites involved in the glycolysis pathway, related to the activation of the Warburg effect, point at virus-induced energy production. For Ad2 proteins, 32 novel phosphorylation sites are identified and as many as 52 phosphorylated sites on 17 different Ad2 proteins are quantified, most of them at late time post-infection. Kinase predictions highlighted activation of PKA, CDK1/2, MAPK, and CKII. Overlaps of kinase motif sequences for viral and human proteins are observed, stressing the importance of phosphorylation during Ad2 infection. |
| Databáze: | OpenAIRE |
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