Prevalence of icaA and icaD Genes of Staphylococcus aureus and Staphylococcus epidermidis Strains Isolated from Patients and Hospital Staff
| Autor: | L. E. Alcaráz, Sara E. Satorres |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Staphylococcus aureus
Micrococcaceae Patients ICAD Virulence medicine.disease_cause Polymerase Chain Reaction law.invention Microbiology Staphylococcus epidermidis law medicine Humans Polymerase chain reaction Base Sequence biology business.industry Public Health Environmental and Occupational Health Biofilm Congo Red General Medicine Staphylococcal Infections biology.organism_classification Culture Media Personnel Hospital Biofilms business Cell Adhesion Molecules Bacteria |
| Zdroj: | Central European Journal of Public Health. 15:87-90 |
| ISSN: | 1803-1048 1210-7778 |
| Popis: | Staphylococci are ubiquitous microorganisms that predominate in normal skin and mucosal flora. Staphylococcus aureus and Staphylococcus epidermidis have been identified as a major cause of nosocomial infections, especially in patients with predisposing factors such as indwelling or implanted foreign bodies. The ability of both S. epidermidis and S. aureus to produce biofilm was compared between 116 clinically significant strains (46 from blood cultures of patients with bloodstream infection and 70 isolated from catheters) and 60 strains isolated from nasal swabs of healthy carriers from hospital staff. The presence of the intercellular adhesion genes (icaA and icaD) was determined by the Polymerase Chain Reaction method, and slime production was examined using qualitative Congo red agar technique. Among clinical strains, 35.2% (19/54) of S. aureus and 48.4% (30/62) of S.epidermidis were both positive icaA and icaD and they produced slime. Among carrier strains, 22.2% (8/36) of S. aureus and 33.3% (8/24) of S. epidermidis were positive for slime synthesis and exhibited ica genes. Our results suggest that the virulence factors contributing to the development of infections can be present in patient and hospital staff isolates. Thus, we consider it is important to detect healthy carriers of slime-producing staphylococci and to control the dissemination of these microorganisms especially in a hospital. |
| Databáze: | OpenAIRE |
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