The Drosophila Translational Control Element (TCE) Is Required for High-Level Transcription of Many Genes That Are Specifically Expressed in Testes
| Autor: | Ashley K. Anderson, Elizabeth A. Rach, David A. Wassarman, Uwe Ohler, Rebeccah J. Katzenberger |
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| Rok vydání: | 2012 |
| Předmět: |
Male
Transcription Genetic Response element Genes Insect RNA polymerase II Regulatory Sequences Nucleic Acid Animals Genetically Modified 0302 clinical medicine Transcription (biology) Nucleic Acids Testis Molecular Cell Biology Transcriptional regulation Drosophila Proteins Promoter Regions Genetic Genetics 0303 health sciences Multidisciplinary Reverse Transcriptase Polymerase Chain Reaction Systems Biology Drosophila Melanogaster Cell Differentiation Animal Models Medicine Female Transcription factor II D Sequence Analysis Research Article Science DNA transcription Biology Cell Line Molecular Genetics 03 medical and health sciences Model Organisms Sequence Homology Nucleic Acid Animals Gene Regulation Gene 030304 developmental biology Base Sequence Evolutionary Developmental Biology Gene Expression Profiling Computational Biology Promoter Molecular Development Gene Expression Regulation Protein Biosynthesis Mutation Transcription Factor TFIID biology.protein Gene expression 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | PLoS ONE, Vol 7, Iss 9, p e45009 (2012) PLoS ONE |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0045009 |
| Popis: | To investigate the importance of core promoter elements for tissue-specific transcription of RNA polymerase II genes, we examined testis-specific transcription in Drosophila melanogaster. Bioinformatic analyses of core promoter sequences from 190 genes that are specifically expressed in testes identified a 10 bp A/T-rich motif that is identical to the translational control element (TCE). The TCE functions in the 5′ untranslated region of Mst(3)CGP mRNAs to repress translation, and it also functions in a heterologous gene to regulate transcription. We found that among genes with focused initiation patterns, the TCE is significantly enriched in core promoters of genes that are specifically expressed in testes but not in core promoters of genes that are specifically expressed in other tissues. The TCE is variably located in core promoters and is conserved in melanogaster subgroup species, but conservation dramatically drops in more distant species. In transgenic flies, short (300–400 bp) genomic regions containing a TCE directed testis-specific transcription of a reporter gene. Mutation of the TCE significantly reduced but did not abolish reporter gene transcription indicating that the TCE is important but not essential for transcription activation. Finally, mutation of testis-specific TFIID (tTFIID) subunits significantly reduced the transcription of a subset of endogenous TCE-containing but not TCE-lacking genes, suggesting that tTFIID activity is limited to TCE-containing genes but that tTFIID is not an obligatory regulator of TCE-containing genes. Thus, the TCE is a core promoter element in a subset of genes that are specifically expressed in testes. Furthermore, the TCE regulates transcription in the context of short genomic regions, from variable locations in the core promoter, and both dependently and independently of tTFIID. These findings set the stage for determining the mechanism by which the TCE regulates testis-specific transcription and understanding the dual role of the TCE in translational and transcriptional regulation. |
| Databáze: | OpenAIRE |
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