A Novel Endosomal Sorting Complex Required for Transport (ESCRT) Component in Arabidopsis thaliana Controls Cell Expansion and Development
| Autor: | Francisca C. Reyes, Rafael Andrade Buono, Li Huey Yeun, Simone Di Rubbo, Eugenia Russinova, Marisa S. Otegui, Hannetz Roschzttardtz |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Endosome Molecular Sequence Data education Arabidopsis Plant Development Saccharomyces cerevisiae macromolecular substances Plasma protein binding Biochemistry ESCRT Species Specificity Arabidopsis thaliana Amino Acid Sequence Gene Silencing Molecular Biology Cell Proliferation Adenosine Triphosphatases Vacuolar protein sorting Endosomal Sorting Complexes Required for Transport biology Arabidopsis Proteins Multivesicular Bodies Cell Biology biology.organism_classification humanities Protein Structure Tertiary Cell biology Plant Leaves Membrane protein Gene Knockdown Techniques Mutation Carrier Proteins Cytokinesis Protein Binding |
| Zdroj: | Journal of Biological Chemistry. 289:4980-4988 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m113.529685 |
| Popis: | ESCRT proteins mediate membrane remodeling and scission events and are essential for endosomal sorting of plasma membrane proteins for degradation. We have identified a novel, plant-specific ESCRT component called PROS (POSITIVE REGULATOR OF SKD1) in Arabidopsis thaliana. PROS has a strong positive effect on the in vitro ATPase activity of SKD1 (also known as Vacuolar Protein Sorting 4 or VPS4), a critical component required for ESCRT-III disassembly and endosomal vesiculation. PROS interacts with both SKD1 and the SKD1-positive regulator LIP5/VTA1. We have identified a putative MIM domain within PROS that mediate the interaction with the MIT domain of SKD1. Interestingly, whereas MIM domains are commonly found at the C terminus of ESCRT-III subunits, the PROS MIM domain is internal. The heterologous expression of PROS in yeast mutant cells lacking Vta1p partially rescues endosomal sorting defects. PROS is expressed in most tissues and cells types in Arabidopsis thaliana. Silencing of PROS leads to reduced cell expansion and abnormal organ growth. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|