Circular RNA Sequencing of Maternal Platelets: A Novel Tool for the Identification of Pregnancy-Specific Biomarkers
| Autor: | Remco Keijser, Erik A. Sistermans, Daoud Sie, Naomi Min, Eva Pajkrt, Joyce Mulders, Aldo Jongejan, Ankie Poutsma, Di-Jan Wigman, Tarah van den Berkmortel, Thomas Wurdinger, Allerdien Visser, Cees B.M. Oudejans, Vera Manders, Myron G. Best, Gijs B. Afink, Marjon A. de Boer, Britt Blanken |
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| Přispěvatelé: | Reproductive Biology Laboratory, Epidemiology and Data Science, APH - Methodology, Obstetrics and Gynaecology, APH - Personalized Medicine, APH - Quality of Care, Amsterdam Reproduction & Development (AR&D), Neurosurgery, Clinical chemistry, ACS - Atherosclerosis & ischemic syndromes, VU University medical center, Obstetrics and gynaecology, Human genetics |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Untranslated region Adult Blood Platelets Pregnancy Tests Clinical Biochemistry Biology Proof of Concept Study Andrology Transcriptome 03 medical and health sciences Exon 0302 clinical medicine Circular RNA medicine Humans circRNA platelet NRIP1 Sequence Analysis RNA Gene Expression Profiling Biochemistry (medical) Trophoblast Placentation RNA RNA sequencing RNA Circular Gene expression profiling Pregnancy Trimester First 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Female pregnancy first trimester Biomarkers |
| Zdroj: | Oudejans, C, Manders, V, Visser, A, Keijser, R, Min, N, Poutsma, A, Mulders, J, van den Berkmortel, T, Wigman, D-J, Blanken, B, Jongejan, A, Pajkrt, E, de Boer, M, Sistermans, E A, Sie, D, Best, M G, Würdinger, T & Afink, G 2021, ' Circular RNA Sequencing of Maternal Platelets: A Novel Tool for the Identification of Pregnancy-Specific Biomarkers ', Clinical Chemistry, vol. 67, no. 3, pp. 508-517 . https://doi.org/10.1093/clinchem/hvaa249 Clinical chemistry, 67(3), 508-517. American Association for Clinical Chemistry Inc. Clinical Chemistry, 67(3), 508-517 |
| ISSN: | 0009-9147 |
| DOI: | 10.1093/clinchem/hvaa249 |
| Popis: | Background In the first trimester of pregnancy, the maternal platelet is directly involved in a positive feedback mechanism that facilitates invasion of the extravillous trophoblast into the maternal spiral arteries. Dysfunctional trophoblast invasion with defective deep placentation is primordial in the etiology of the “great obstetrical syndromes.” Methods In this proof-of-concept study, using transcriptome analysis of circular RNA (circRNA) following RNA sequencing of maternal platelets, we tested whether pregnancy-specific circRNA markers could be identified in the first trimester of normal pregnancies. Differential transcript expression analysis of circRNAs, as predicted by Accurate CircRNA Finder Suite, CircRNA Identifier (version 2), and Known and Novel Isoform Explorer, was done using thromboSeq.R with variation of multiple settings. Test performance was checked for (a) de novo circRNA identification using the novel platelet-specific Plt-circR4 as a positive control, (b) complete segregation of groups (pregnant vs nonpregnant) after heat map–dendrogram clustering, (c) identification of pregnancy-specific circRNA markers at a false discovery rate (FDR) Results Of the differentially expressed circRNAs with P values 2), and 52 circRNAs were downregulated (logFC less than −2) in first-trimester platelet RNA. Of these, nuclear receptor-interacting protein 1 circRNA covering exons 2 and 3 of the 5′-untranslated region was pregnancy specific with upregulation in first-trimester maternal platelets compared to nonpregnant controls. Conclusion CircRNA sequencing of first-trimester maternal platelets permits the identification of novel pregnancy-specific RNA biomarkers. Future use could include the assessment of maternal and fetal well-being. |
| Databáze: | OpenAIRE |
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