Dimethylation of Histone 3 Lysine 9 is sensitive to the epileptic activity, and affects the transcriptional regulation of the potassium channel Kcnj10 gene in epileptic rats
Autor: | Hong Tao, Tao Sun, Lin‑Na Zhang, Man Zhang, Chun‑Hui Wang, Yan Luo, Tao He, Qi‑Kuan Hu, Ling Chen, Shao‑Ping Zhang, Xiao‑Cheng Li |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Male Cancer Research Gene Expression KCNJ10 Biology Biochemistry Hippocampus Methylation Epigenesis Genetic Histones Rats Sprague-Dawley 03 medical and health sciences Epilepsy 0302 clinical medicine Cell Line Tumor Gene expression Genetics Transcriptional regulation medicine Gene silencing Animals Potassium Channels Inwardly Rectifying Promoter Regions Genetic Molecular Biology Inward-rectifier potassium ion channel Promoter Histone-Lysine N-Methyltransferase medicine.disease Molecular biology Potassium channel 030104 developmental biology Oncology biology.protein Molecular Medicine 030217 neurology & neurosurgery Protein Binding |
Zdroj: | Molecular medicine reports. 17(1) |
ISSN: | 1791-3004 |
Popis: | Potassium channels can be affected by epileptic seizures and serve a crucial role in the pathophysiology of epilepsy. Dimethylation of histone 3 lysine 9 (H3K9me2) and its enzyme euchromatic histone‑lysine N‑methyltransferase 2 (G9a) are the major epigenetic modulators and are associated with gene silencing. Insight into whether H3K9me2 and G9a can respond to epileptic seizures and regulate expression of genes encoding potassium channels is the main purpose of the present study. A total of 16 subtypes of potassium channel genes in pilocarpine‑modelled epileptic rats were screened by reverse transcription‑quantitative polymerase chain reaction, and it was determined that the expression ATP‑sensitive inward rectifier potassium channel 10 (Kcnj10) increased in hippocampus and insular cortex, while the expression of most of the other subtypes decreased. The total level of H3K9me2 decreased in the model group compared with the control. The Kcnj10 gene encoding the Kir4.1 channel was selected to analyse changes in H3K9me2 in the promoter region by the chromatin immuno‑precipitation method. Anti‑H3K9me2 and anti‑G9a antibodies were used to identify the modified DNAs. Five primers were designed across the promoter region of the Kcnj10 gene. In epileptic hippocampi, the relative abundance of H3K9me2 and G9a in the promoter region of Kcnj10 decreased markedly. Removal of the H3K9me2 repressive mark resulted in decreased transcriptional inhibition of the Kcnj10 gene and therefore increased its expression. In the cultured C6 cells, specific inhibition of the enzymatic activity of G9a by 2‑(Hexahydro‑4‑methyl‑1H‑1,4‑diazepin‑1‑yl)‑6,7‑di‑ methoxy‑N‑(1‑(phenyl‑methyl)‑4‑piperidinyl)‑4‑quinazolinamine tri‑hydrochloride hydrate (bix01294) resulted in upregulation of the expression of Kir4.1 proteins. The present study demonstrated that H3K9me2 and G9a are sensitive to epileptic seizure activity during the acute phase of epilepsy and can affect the transcriptional regulation of the Kcnj10 channel. |
Databáze: | OpenAIRE |
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