Characteristics of Escherichia coli sequence type 131 isolates that produce extended-spectrum β-lactamases: global distribution of the H30-Rx sublineage
| Autor: | Gisele Peirano, Michael Costello, Patrice Nordmann, Joshua L. Freeman, Laurent Poirel, Akke K. van der Bij, Johann D. D. Pitout, Veronika Tchesnokova |
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| Rok vydání: | 2014 |
| Předmět: |
DNA Topoisomerase IV
Lineage (genetic) Drug resistance Microbial Sensitivity Tests Biology medicine.disease_cause DNA gyrase beta-Lactamases Microbiology Epidemiology and Surveillance Plasmid Drug Resistance Bacterial medicine Escherichia coli Humans Pharmacology (medical) Bacteriophage Typing Escherichia coli Infections Phage typing Pharmacology Adhesins Escherichia coli Phylogenetic tree Multiple drug resistance Infectious Diseases DNA Gyrase Fimbriae Proteins Plasmids |
| Zdroj: | Antimicrobial agents and chemotherapy. 58(7) |
| ISSN: | 1098-6596 |
| Popis: | We designed a study to describe the characteristics of sequence type 131 (ST131) lineages, including the H 30-Rx sublineage, among a global collection of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates from 9 countries collected from 2000 to 2011. A total of 240 nonrepeat isolates from Canada, the United States, Brazil, the Netherlands, France, the United Arab Emirates (UAE), India, South Africa, and New Zealand were included. Established PCR, sequencing, and typing methods were used to define ST131 lineages, H 30 and H 30-Rx phylogenetic groups, gyrA and parC mutations, virotypes, and plasmid-mediated quinolone resistance determinants. The majority of the isolates produced CTX-M-15 with aac(6′)-lb-cr , belonged to phylogenetic group B2, and were positive for the H 30 lineage with the gyrA1AB and parC1aAB mutations. ST131 showed 15 distinct pulsotypes; 43% of the isolates belonged to four pulsotypes, with a global distribution. Seventy-five percent of the ST131 isolates belonged to H 30-Rx; this sublineage was present in all the countries and was associated with multidrug resistance, bla CTX-M-15 , aac(6′)-lb-cr , and virotypes A and C. The H 41 lineage was negative for the ST131 pabB allele-specific PCR. The multidrug-resistant H 30-Rx sublineage poses an important public health threat due to its global distribution, association with virotype C, and high prevalence among ST131 isolates that produce CTX-M-15. |
| Databáze: | OpenAIRE |
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