Imaging of Endogenous Metabolites of Plant Leaves by Mass Spectrometry Based on Laser Activated Electron Tunneling
| Autor: | Hongying Zhong, Lulu Huang, Xuemei Tang, Wenyang Zhang, Juan Zhang, Ruowei Jiang, Disong Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Matrix-assisted laser desorption electrospray ionization Materials science Analytical chemistry Metal Nanoparticles Thermal ionization Electrons Cyclopentanes Mass spectrometry 01 natural sciences Citric Acid Article Atmospheric-pressure laser ionization 03 medical and health sciences Ionization Metabolomics Oxylipins Electron ionization Multidisciplinary Lasers Fatty Acids 010401 analytical chemistry Oxides Cobalt Gibberellins Ion source 0104 chemical sciences Plant Leaves 030104 developmental biology Semiconductors Vitaceae Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Field desorption Zinc Oxide Crystallization Bismuth Abscisic Acid |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep24164 |
| Popis: | A new mass spectrometric imaging approach based on laser activated electron tunneling (LAET) was described and applied to analysis of endogenous metabolites of plant leaves. LAET is an electron-directed soft ionization technique. Compressed thin films of semiconductor nanoparticles of bismuth cobalt zinc oxide were placed on the sample plate for proof-of-principle demonstration because they can not only absorb ultraviolet laser but also have high electron mobility. Upon laser irradiation, electrons are excited from valence bands to conduction bands. With appropriate kinetic energies, photoexcited electrons can tunnel away from the barrier and eventually be captured by charge deficient atoms present in neutral molecules. Resultant unpaired electron subsequently initiates specific chemical bond cleavage and generates ions that can be detected in negative ion mode of the mass spectrometer. LAET avoids the co-crystallization process of routinely used organic matrix materials with analyzes in MALDI (matrix assisted-laser desorption ionization) analysis. Thus uneven distribution of crystals with different sizes and shapes as well as background peaks in the low mass range resulting from matrix molecules is eliminated. Advantages of LAET imaging technique include not only improved spatial resolution but also photoelectron capture dissociation which produces predictable fragment ions. |
| Databáze: | OpenAIRE |
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