The comparative release of FGF1 by hypoxia and temperature stress
| Autor: | Mouta Carreira C, Igor Prudovsky, Matteo Landriscina, Thomas Maciag, Stephen Bellum |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Angiogenesis
Macromolecular Substances Clinical Biochemistry Aminopyridines Nerve Tissue Proteins Biology Fibroblast growth factor SYT1 Transfection Synaptotagmin 1 Cell Line Lipoxygenase Mice Synaptotagmins Endocrinology Stress Physiological medicine Animals Humans Cyclooxygenase Inhibitors Cysteine Lipoxygenase Inhibitors Nitrobenzenes Sulfonamides Arachidonic Acid Membrane Glycoproteins Calcium-Binding Proteins Temperature Cell Biology 3T3 Cells Hypoxia (medical) FGF1 5 8 11 14-Eicosatetraynoic Acid Cell Hypoxia Cell biology Cell culture Synaptotagmin I biology.protein Fibroblast Growth Factor 1 medicine.symptom Dimerization |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0897-7194 |
| Popis: | The signal peptide-less FGF gene family prototype, FGF1 is released in response to temperature stress in vitro as a latent reducing agent-sensitive homodimer non-covalently complexed with the extravesicular p40 domain of p65 synaptotagmin (Syt)1. Because FGF1 is well recognized as an angiogenesis factor in vivo and angiogenesis is known to be induced by hypoxia, we examined the release of FGF1 and p40 Syt1 under conditions of hypoxia and temperature stress using a chemostatic microcarrier cell culture system. We report that like the pathway used by FGF1 and p40 Syt1 release under temperature stress, hypoxia also induces the release of FGF1 and p40 Syt1 with similar kinetic and pharmacologic properties including the requirement for functional cysteine residues. Lastly, FGF1 and p40 Syt1 release in response to hypoxia and temperature stress is sensitive to lipoxygenase and cyclooxygenase inhibitors suggesting that arachidonic acid metabolism may play an important role in the mechanism of FGF1 releas... |
| Databáze: | OpenAIRE |
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