Interleukin-2 differentially regulates IL-2 receptors on murine cloned cytolytic and helper T cells
| Autor: | Wendy L. Havran, Do-Kyun Kim, David W. Lancki, R. L. Moldwin, Frank W. Fitch |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Interleukin 2
medicine.medical_specialty Time Factors Helper T lymphocyte Receptor expression Immunology Biology Pathology and Forensic Medicine Mice Cell surface receptor Internal medicine Concanavalin A medicine Animals Immunology and Allergy Receptors Immunologic Receptor Dose-Response Relationship Drug Lymphokine Antibodies Monoclonal Receptors Interleukin-2 T-Lymphocytes Helper-Inducer T lymphocyte Molecular biology Recombinant Proteins Clone Cells Cortisone Endocrinology Cell culture Interleukin-2 Spleen T-Lymphocytes Cytotoxic medicine.drug |
| Zdroj: | Clinical Immunology and Immunopathology. 39:368-378 |
| ISSN: | 0090-1229 |
| DOI: | 10.1016/0090-1229(86)90165-0 |
| Popis: | The effects of interleukin-2 (IL-2) on the expression of IL-2 receptors by cloned cytolytic and helper T lymphocytes were studied using three anti-IL-2 receptor antibodies. IL-2 enhanced the expression of IL-2 receptors on all clones tested. A steep dose-response curve was observed with no measurable effect seen below 2 units/ml and maximal IL-2 receptor expression with greater than 5 units/ml. IL-2 receptor expression peaked 24–48 hr after the addition of IL-2. The subsequent decrease in IL-2 receptor expression correlated with a decrease in the levels of IL-2 remaining in the culture supernatants of cytolytic T lymphocyte cells. Removing residual IL-2 from cultures resulted in the rapid return of IL-2 receptor expression to unstimulated levels. The daily addition of low levels of IL-2 to cultures resulted in the prolonged expression of high levels of IL-2 receptors by non-IL-2-producing cloned cytolytic T cells. Cloned helper T cells which make IL-2 showed the initial increase in IL-2 receptor levels, but the daily addition of IL-2 did not prolong IL-2 receptor expression in these cells. These data suggest that IL-2 receptors on those cells which do not make IL-2 are regulated differently from receptors on cells which themselves make IL-2. |
| Databáze: | OpenAIRE |
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