CCL27 expression is regulated by both p38 MAPK and IKKβ signalling pathways
| Autor: | Knud Kragballe, Claus Johansen, Lars Iversen, Jette Lindorff Riis, Christian Vestergaard, Kristian Otkjaer |
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| Rok vydání: | 2010 |
| Předmět: |
MAPK/ERK pathway
Keratinocytes MAP Kinase Signaling System Pyridines p38 mitogen-activated protein kinases Immunology Interleukin-1beta Molecular Sequence Data IκB kinase Thiophenes Biochemistry Ribosomal Protein S6 Kinases 90-kDa p38 Mitogen-Activated Protein Kinases Chemokine receptor Immunology and Allergy Humans CCR10 Protein kinase A Promoter Regions Genetic Molecular Biology Cation Transport Proteins Protein Kinase Inhibitors Adenosine Triphosphatases Binding Sites biology Base Sequence Kinase Chemokine CCL27 Imidazoles Transcription Factor RelA Hematology Cell biology I-kappa B Kinase Epidermal Cells Gene Expression Regulation Copper-Transporting ATPases Mitogen-activated protein kinase biology.protein Protein Multimerization Protein Binding |
| Zdroj: | Riis, J L, Johansen, C, Vestergaard, C, Otkjaer, K, Kragballe, K & Iversen, L 2011, ' CCL27 expression is regulated by both p38 MAPK and IKKβ signalling pathways ', Cytokine Yearbook . https://doi.org/10.1016/j.cyto.2011.09.007 |
| ISSN: | 1096-0023 |
| DOI: | 10.1016/j.cyto.2011.09.007 |
| Popis: | The skin-specific chemokine CCL27 is believed to play a pivotal role in establishing the inflammatory infiltrate characteristic for common inflammatory skin diseases. Through binding to the chemokine receptor 10 (CCR10), CCL27 mediates inflammation by promoting lymphocyte migration into the skin. Little is known about the regulation of CCL27 gene expression. The purpose of our study was to investigate the regulation of the IL-1β-induced CCL27 gene expression in normal human keratinocytes (NHEK). Preincubation of NHEK with the inhibitory κB (IκB) kinase (IKK) inhibitor, SC-514, or the p38 mitogen-activated protein kinase (MAPK) inhibitor, SB202190, revealed a profound reduction in both CCL27 mRNA and CCL27 protein expression indicating the significance of these pathways in the regulation of CCL27 expression. Furthermore, the impact of inhibitors of mitogen- and stress-activated kinase 1 (MSK1) or the mitogen-activated protein kinase-interacting kinases (Mnk1 + 2), downstream kinases of p38 MAPK, on IL-1β-induced CCL27 expression in NHEK were investigated. We identified seven NF-κB binding elements upstream from the CCL27 gene start codon using electrophoretic mobility shift assay (EMSA). Supershift analyses demonstrated the involvement of the p50/p65 NF-κB heterodimer. We conclude that IL-1β-induced CCL27 gene expression in NHEK is regulated through the p38 MAPK/MSK1/Mnk1 + 2 as well as the IKKβ/NF-κB signalling pathways. |
| Databáze: | OpenAIRE |
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